Evidence map›Paper›PMID 40557276›Full record

ArticlemicroPublication biology2025

Rap1 activity and localization is regulated by Rab40/CRL5 facilitated mono-ubiquitylation.

Andrew Neumann, Revathi Sampath, Ke-Jun Han, Rytis Prekeris

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in microPublication biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Andrew NeumannCell and Developmental Biology, University of Colorado Anschutz Medical Campus, Aurora, Colorado, United States.
Revathi SampathCell and Developmental Biology, University of Colorado Anschutz Medical Campus, Aurora, Colorado, United States.
Ke-Jun HanCell and Developmental Biology, University of Colorado Anschutz Medical Campus, Aurora, Colorado, United States.
Rytis PrekerisCell and Developmental Biology, University of Colorado Anschutz Medical Campus, Aurora, Colorado, United States.

Funding

Predoctoral Training Program in Molecular and Cellular Biology (Supplement: Mentoring in the Research Environment)T32GM136444 · NIGMS · UNIVERSITY OF COLORADO DENVER · PI MICHAEL A MCMURRAY, Rytis Prekeris · 2020 to 2026
$3.7M
The mechanisms regulating actin dynamics and polarized membrane transport during invadopodia formationR01GM122768 · NIGMS · UNIVERSITY OF COLORADO DENVER · PI PREKERIS, RYTIS · 2018 to 2025
$2.6M
NIGMS NIH HHS R01 GM122768NIGMS NIH HHS T32 GM136444
6 · The paper itself

Abstract

The Rap family of GTPases are emerging as major regulators of actin dynamics and cell migration. However, how Rap GTPases are activated and targeted to their subcellular localization remains to be fully understood. Recent work has shown that Rab40/CRL5-dependent mono-ubiquitylation is required for Rap2 activation. Here, we show that Rap1 is also mono-ubiquitylated by a Rab40/CRL5 E3 ubiquitin ligase complex and that Rap1 mono-ubiquitylation is necessary for Rap1 localization to both the plasma membrane and nuclear envelope. In summary, this work shows that Rab40/CRL5 is a major regulator of the activity and spatiotemporal dynamics of the Rap family of GTPases.

Identifiers

PMID40557276
PMCPMC12186120

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.