Evidence map›Paper›PMID 40556020›Full record

ArticleJournal of animal science and biotechnology2025

Circ-0000197 derived from porcine milk small extracellular vesicles promotes intestinal barrier function by sponging miR-429.

Yuxuan Wang, Bilan Chen, Tingzhou Xuan, Kun Ouyang, Jingshen Chen, Hailong Wang, Junyi Luo, Jiajie Sun, Qianyun Xi, Yongliang Zhang and 1 more

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Article in Journal of animal science and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

11 authors.

Yuxuan Wang *College of Animal Science, Guangdong Province Key Laboratory of Animal Nutritional Regulation, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Livestock and Poultry Breeding, South China Agricultural University, Guangzhou, 510642, Guangdong, China.
Bilan Chen *College of Animal Science, Guangdong Province Key Laboratory of Animal Nutritional Regulation, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Livestock and Poultry Breeding, South China Agricultural University, Guangzhou, 510642, Guangdong, China.
Tingzhou XuanCollege of Animal Science, Guangdong Province Key Laboratory of Animal Nutritional Regulation, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Livestock and Poultry Breeding, South China Agricultural University, Guangzhou, 510642, Guangdong, China.
Kun OuyangCollege of Animal Science, Guangdong Province Key Laboratory of Animal Nutritional Regulation, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Livestock and Poultry Breeding, South China Agricultural University, Guangzhou, 510642, Guangdong, China.
Jingshen ChenCollege of Animal Science, Guangdong Province Key Laboratory of Animal Nutritional Regulation, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Livestock and Poultry Breeding, South China Agricultural University, Guangzhou, 510642, Guangdong, China.
Hailong WangCollege of Animal Science, Guangdong Province Key Laboratory of Animal Nutritional Regulation, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Livestock and Poultry Breeding, South China Agricultural University, Guangzhou, 510642, Guangdong, China.
Junyi LuoCollege of Animal Science, Guangdong Province Key Laboratory of Animal Nutritional Regulation, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Livestock and Poultry Breeding, South China Agricultural University, Guangzhou, 510642, Guangdong, China.
Jiajie SunCollege of Animal Science, Guangdong Province Key Laboratory of Animal Nutritional Regulation, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Livestock and Poultry Breeding, South China Agricultural University, Guangzhou, 510642, Guangdong, China.
Qianyun XiCollege of Animal Science, Guangdong Province Key Laboratory of Animal Nutritional Regulation, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Livestock and Poultry Breeding, South China Agricultural University, Guangzhou, 510642, Guangdong, China.
Yongliang ZhangCollege of Animal Science, Guangdong Province Key Laboratory of Animal Nutritional Regulation, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Livestock and Poultry Breeding, South China Agricultural University, Guangzhou, 510642, Guangdong, China. zhangyl@scau.edu.cn.
Ting ChenCollege of Animal Science, Guangdong Province Key Laboratory of Animal Nutritional Regulation, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Livestock and Poultry Breeding, South China Agricultural University, Guangzhou, 510642, Guangdong, China. allinchen@scau.edu.cn.

Funding

National key research and development program 2022YFD1300401National Natural Science Foundation of China 32372958Natural Science Foundation of Guangdong Province 2023A1515012127Natural Science Foundation of Guangdong Province 2024A1515010510Natural Science Foundation of Guangdong Province 2025A1515011832
6 · The paper itself

Abstract

backgroundAs an essential source of nutrients for young mammals, milk possesses a variety of biological functions. Recently identified milk-derived small extracellular vesicles (sEV) have shown potential regulatory effects on intestinal health. Current studies have highlighted the functional roles of milk-derived sEV and their RNA cargo in promoting intestinal health. However, there is a paucity of research demonstrating how milk-derived sEV influence intestinal barrier function through the transport of circRNAs.

resultsIn this study, we aimed to investigate the effects of porcine milk sEV (PM-sEV) circRNA on intestinal barrier function. We systematically identified the circRNAs involved in this process and analyzed the miRNAs through which PM-sEV deliver circRNAs to regulate intestinal barrier function. Our findings revealed that PM-sEV promote the expression of the intestinal tight junction proteins ZO-1 and Occludin, both in vivo (mice) and in vitro (IPEC-J2). When PM-sEV RNA was reduced using ultrasound treatment, their ability to enhance intestinal barrier function was significantly reduced. Bioinformatics analysis showed that circ-0000197, present in PM-sEV, can target miR-429, while miR-429 has the ability to target the 3'-UTR of ZO-1 and Occludin. Furthermore, experiments involving the overexpression or inhibition of the relevant non-coding RNAs (ncRNAs) demonstrated that circ-0000197 significantly enhances intestinal barrier function, whereas miR-429 exerts an inhibitory effect on this function. Overall, our findings identify circ-0000197 in PM-sEV as a crucial circRNA that regulates intestinal barrier function by inhibiting miR-429. Circ-0000197 carried by PM-sEV acts as a competing endogenous RNA (ceRNA) that regulates the expression of ZO-1 and Occludin by sponging miR-429, thereby promoting intestinal barrier function at both the cellular and in vivo levels.

conclusionsThese findings emphasize the vital role of circRNAs transported through milk-derived sEV in regulating intestinal health, offering new avenues for developing innovative functional milk components. This mechanism also underscores the importance of PM-sEV carrying circ-0000197 in preserving intestinal barrier integrity. Collectively, this study enhances our understanding of the complex regulatory networks involving PM-sEV carrying circRNAs and their impact on intestinal health.

Indexed as

CircRNAIntestinal barrierMilk small extracellular vesiclesMiRNAOccludinZO-1

Identifiers

PMID40556020
PMCPMC12188651

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.