Evidence map›Paper›PMID 40555934›Full record

ArticleWorld journal of microbiology & biotechnology2025

Enhanced L-serine production in Corynebacterium glutamicum via promoter engineering and Bayesian optimization.

Yujie Gao, Anqian Liu, Xiaomei Zhang, Wei Zhang, Xiaogang Wang, Guoqiang Xu, Xiaojuan Zhang, Hui Li, Jinsong Shi, Zhenghong Xu

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Article in World journal of microbiology & biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Yujie GaoBiotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.
Anqian LiuLaboratory of Pharmaceutical Engineering, School of Life Science and Health Engineering, Jiangnan University, Wuxi, 214122, China.
Xiaomei ZhangLaboratory of Pharmaceutical Engineering, School of Life Science and Health Engineering, Jiangnan University, Wuxi, 214122, China. zhangxiaomei@jiangnan.edu.cn.
Wei ZhangLaboratory of Pharmaceutical Engineering, School of Life Science and Health Engineering, Jiangnan University, Wuxi, 214122, China.
Xiaogang WangKey Laboratory of Advanced Control for Light Industry Processes, Ministry of Education, Jiangnan University, Wuxi, 214122, Jiangsu, China.
Guoqiang XuBiotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.
Xiaojuan ZhangBiotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.
Hui LiLaboratory of Pharmaceutical Engineering, School of Life Science and Health Engineering, Jiangnan University, Wuxi, 214122, China.
Jinsong ShiLaboratory of Pharmaceutical Engineering, School of Life Science and Health Engineering, Jiangnan University, Wuxi, 214122, China.
Zhenghong XuBiotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China. zhenghxu@scu.edu.cn.

Funding

National Natural Science Foundation of China 32171470
6 · The paper itself

Abstract

L-serine is a valuable compound in various industries, including medicine, food and cosmetics. Corynebacterium glutamicum is the preferred choice for the fermentation production of amino acids. In this study, to enhance the L-serine titer, we engineered a C. glutamicum strain capable of high production of L-serine by regulating the transcription level of the L-serine synthesis key enzymes (serA, serC and serB). Firstly, the native promoter strengths of serA, serC and serB in strain C. glutamicum A36 (with 31.1 g/L L-serine titer) were evaluated and compared with four known-strength promoters (Pkan, dap-e, A16-1 and dap-e11). The promoter strength ratios were P-serA: P-serC: P-serB = 101.70: 1: 7.69, ranking as P-serC < Pkan < P-serB < dap-e < P-serA < A16-1 < dap-e11. Then, we regulated and enhanced the L-serine synthesis pathway by promoter engineering through two steps. In the first step, the native promoters of serA, serC and serB were replaced with stronger promoters individually, leading to 9 recombinant strains. The highest L-serine titer of A36-serA

Indexed as

Corynebacterium glutamicumMetabolic EngineeringPromoter Regions, GeneticSerineBacterial ProteinsBayes TheoremBiosynthetic PathwaysFermentationGene Expression Regulation, BacterialBacterial ProteinsSerineBayesian optimizationCorynebacterium glutamicumL-serineL-serine synthesisPromoter

Identifiers

PMID40555934

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.