Evidence map›Paper›PMID 40555232›Full record

ArticleCell reports methods2025

Characterization of human CMV-specific CD8

Ioanna Gemünd, Lorenzo Bonaguro, Matthias Becker, Sophie Müller, Clemens Joos, Elena De Domenico, Anna C Aschenbrenner, Joachim L Schultze, Andreas Moosmann, Marc D Beyer

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports methods, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Ioanna GemündSystems Medicine, German Center for Neurodegenerative Diseases (DZNE), Bonn, Germany; Genomics and Immunoregulation, Life and Medical Sciences (LIMES) Institute, University of Bonn, Bonn, Germany; Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Parkville, VIC, Australia; Institute of Virology, Technical University of Munich School of Medicine/Helmholtz Munich, Munich, Germany.
Lorenzo BonaguroSystems Medicine, German Center for Neurodegenerative Diseases (DZNE), Bonn, Germany; PRECISE Platform for Single Cell Genomics and Epigenomics, DZNE, and University of Bonn and West German Genome Center, Bonn, Germany.
Matthias BeckerSystems Medicine, German Center for Neurodegenerative Diseases (DZNE), Bonn, Germany; Modular High-Performance Computing and Artificial Intelligence, DZNE, Bonn, Germany.
Sophie MüllerSystems Medicine, German Center for Neurodegenerative Diseases (DZNE), Bonn, Germany; Genomics and Immunoregulation, Life and Medical Sciences (LIMES) Institute, University of Bonn, Bonn, Germany; Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Parkville, VIC, Australia.
Clemens JoosMedizinische Klinik und Poliklinik III, LMU Klinikum, LMU München, Munich, Germany; Helmholtz Munich, Munich, Germany.
Elena De DomenicoSystems Medicine, German Center for Neurodegenerative Diseases (DZNE), Bonn, Germany; PRECISE Platform for Single Cell Genomics and Epigenomics, DZNE, and University of Bonn and West German Genome Center, Bonn, Germany.
Anna C AschenbrennerSystems Medicine, German Center for Neurodegenerative Diseases (DZNE), Bonn, Germany.
Joachim L SchultzeSystems Medicine, German Center for Neurodegenerative Diseases (DZNE), Bonn, Germany; Genomics and Immunoregulation, Life and Medical Sciences (LIMES) Institute, University of Bonn, Bonn, Germany; PRECISE Platform for Single Cell Genomics and Epigenomics, DZNE, and University of Bonn and West German Genome Center, Bonn, Germany.
Andreas MoosmannMedizinische Klinik und Poliklinik III, LMU Klinikum, LMU München, Munich, Germany; Helmholtz Munich, Munich, Germany; German Center for Infection Research (DZIF), Munich Partner Site, Munich, Germany.
Marc D BeyerSystems Medicine, German Center for Neurodegenerative Diseases (DZNE), Bonn, Germany; PRECISE Platform for Single Cell Genomics and Epigenomics, DZNE, and University of Bonn and West German Genome Center, Bonn, Germany; Immunogenomics & Neurodegeneration, Systems Medicine, DZNE, Bonn, Germany. Electronic address: marc.beyer@dzne.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this study, we established a comprehensive workflow to collect multi-omics single-cell data using a commercially available micro-well-based platform. This included whole transcriptome, cell surface markers (targeted sequencing-based cell surface proteomics), T cell specificities, adaptive immune receptor repertoire (AIRR) profiles, and sample multiplexing. With this technique, we identified paired T cell receptor sequences for three prominent human CMV epitopes. In addition, we assessed the ability of dCODE dextramers to detect antigen-specific T cells at low frequencies by estimating sensitivities and specificities when used as reagents for single-cell multi-omics.

Indexed as

CD8-Positive T-LymphocytesCytomegalovirusProteomicsSingle-Cell AnalysisCytomegalovirus InfectionsHumansReceptors, Antigen, T-CellTranscriptomeReceptors, Antigen, T-Cellantigen-specific T cellsBD RhapsodyCD8(+) T cellsCMVCP: immunologydCODE dextramerssingle-cell multi-omicssystems immunologyTCR sequencing

Identifiers

PMID40555232
PMCPMC12296424

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.