ArticleJournal of animal science2025
Metabolomics of follicular fluid reveals the impact of heat stress on oocyte pick-up and in vitro embryo production efficiency in dairy cows.
Article in Journal of animal science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Melatonin Enhances Embryo Quality in Japanese Black Cows During the Hot Season.Reproduction in domestic animals = Zuchthygiene · 2026Article
- Melatonin Supplementation Increases Oocyte Recovery and Quality Associated with Altered Follicular Steroidogenesis and Redox Status in Dairy Cows.Antioxidants (Basel, Switzerland) · 2026Article
- Rumen-host crosstalk: an amplifier of systemic pathology in heat-stressed ruminants.Frontiers in microbiology · 2026Review
- Six things to know about how heat stress compromises fertility of the cow (and probably other mammals as well).The Journal of reproduction and development · 2026Review
- Unraveling the Molecular Landscape of Follicular Fluid: A Comparative Proteomic Study Between Bali and Brahman Cattle.Veterinary medicine international · 2026Article
- Molecular and developmental consequences of heat stress on the bovine oocyte and embryo competence.Animal reproduction · 2026Article
- Reactive Oxygen Species in Follicular Fluid as a Potential Biomarker of Oocyte Developmental Competence.Journal of reproduction & infertility · 2025Article
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Authors and funding
10 authors.
Funding
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Abstract
Heat stress (HS) is a major challenge in global dairy farming, severely affecting reproductive performance. However, the molecular mechanisms by which it regulates oocyte development by altering the follicular microenvironment are unclear. This study assessed HS-related indicators in summer and autumn, dividing cows into HS and non-HS (TN) groups to evaluate the effect of HS on oocyte pick-up and in vitro embryo production (OPU-IVP) efficiency, and used untargeted metabolomics to analyze its impact on follicular fluid (FF) metabolic characteristics. The results showed that HS significantly affected the physiological and production performance of dairy cows. Serum and FF analysis showed significant changes in oxidative stress indicators (SOD, GSH-Px, CAT, MDA, T-AOC, ROS), inflammatory factors (IL-1, IL-6, TNF-α), heat shock proteins (HSP70 and HSP90), and steroid hormones (E2 and P4) induced by HS. Moreover, HS significantly reduced the total number of oocytes, oocyte recovery rate, proportions of grade A and B oocytes, cleavage rate, and blastocyst rate in OPU-IVP. Untargeted metabolomics analysis revealed 1544 differential metabolites in FF, which were mainly enriched in pathways such as steroid hormone biosynthesis, neuroactive ligand-receptor interactions, D-amino acid metabolism, tyrosine metabolism, phenylalanine metabolism, and tryptophan metabolism. In conclusion, this study not only reveals for the first time the metabolic characteristics of FF under HS and the potential mechanisms affecting oocyte development, but also provides innovative insights for developing precise interventions to improve OPU-IVP efficiency in dairy cows under HS.
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