ArticleBiomacromolecules2025
Reinforcement of Fibrillar Collagen Hydrogels with Bioorthogonal Covalent Crosslinks.
Lucia G Brunel, Chris M Long, Fotis Christakopoulos, Betty Cai, Narelli de Paiva Narciso, Patrik K Johansson, Diya Singhal, Neil J Baugh, Daiyao Zhang, Annika Enejder and 2 more
Abstract read
In one paragraphArticle in Biomacromolecules, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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2 · The registryThe trial behind it
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5 · Who and what moneyAuthors and funding
12 authors.
Lucia G BrunelDepartment of Chemical Engineering, Stanford University, Stanford, California 94305, United States.ORCID 0000-0003-0327-5635 Chris M LongDepartment of Materials Science and Engineering, Stanford University, Stanford, California 94305, United States.
Fotis ChristakopoulosDepartment of Materials Science and Engineering, Stanford University, Stanford, California 94305, United States.ORCID 0000-0001-7383-5875 Betty CaiDepartment of Materials Science and Engineering, Stanford University, Stanford, California 94305, United States.
Narelli de Paiva NarcisoDepartment of Materials Science and Engineering, Stanford University, Stanford, California 94305, United States.
Patrik K JohanssonDepartment of Materials Science and Engineering, Stanford University, Stanford, California 94305, United States.
Diya SinghalDepartment of Chemical Engineering, Stanford University, Stanford, California 94305, United States.
Neil J BaughDepartment of Materials Science and Engineering, Stanford University, Stanford, California 94305, United States.
Daiyao ZhangDepartment of Chemical Engineering, Stanford University, Stanford, California 94305, United States.
Annika EnejderDepartment of Materials Science and Engineering, Stanford University, Stanford, California 94305, United States.ORCID 0000-0003-2000-1353 David MyungDepartment of Chemical Engineering, Stanford University, Stanford, California 94305, United States.
Sarah C HeilshornDepartment of Materials Science and Engineering, Stanford University, Stanford, California 94305, United States.ORCID 0000-0002-9801-6304 Funding
Stanford Vision Research CoreP30EY026877 · NEI · STANFORD UNIVERSITY · PI Jeffrey L Goldberg · 2017 to 2026
$8.0MCorneal Scar Repair through SPAACKL: Sutureless, Pro-regenerative Anterior Additive Collagen gel KeratopLastyR01EY033363 · NEI · STANFORD UNIVERSITY · PI David Myung · 2022 to 2026
$2.5M3D bioprinting of a bilayered, tissue engineered corneaR01EY035697 · NEI · STANFORD UNIVERSITY · PI Sarah C Heilshorn, David Myung · 2024 to 2026
$1.6MLiposome-crosslinked hyaluronic acid hydrogels with localized protein delivery for myocardial infarction therapyF31HL175888 · NHLBI · STANFORD UNIVERSITY · PI Neil Baugh · 2024 to 2026
$130k3D bioprinting of regenerative, corneal cell-laden inks to treat corneal blindnessF31EY034785 · NEI · STANFORD UNIVERSITY · PI BRUNEL, LUCIA · 2023 to 2024
$82kNEI NIH HHS F31 EY034785NEI NIH HHS P30 EY026877NEI NIH HHS R01 EY033363NEI NIH HHS R01 EY035697NHLBI NIH HHS F31 HL175888
6 · The paper itselfAbstract
Bioorthogonal covalent crosslinking stabilizes collagen type I hydrogels, improving their structural integrity for tissue engineering applications with encapsulated living cells. The chemical modification required for crosslinking, however, interferes with the fibrillar nature of the collagen, leading instead to an amorphous network without fibers. We demonstrate an approach to perform bioconjugation chemistry on collagen with controlled localization such that the modified collagen retains its ability to self-assemble into a fibrillar network while also displaying functional groups for covalent crosslinking with bioorthogonal click chemistry. The collagen matrix is formed through a sequential crosslinking process, in which the modified collagen first physically assembles into fibers and then is covalently crosslinked. This approach preserves the fibrous architecture of the collagen, guiding the behavior of encapsulated human corneal mesenchymal stromal cells while also reinforcing fibers through covalent crosslinks, strengthening the stability of the cell-laden collagen hydrogel against cell-induced contraction and enzymatic degradation.
Indexed as
Collagen Type ICross-Linking ReagentsHydrogelsClick ChemistryCorneaHumansMesenchymal Stem CellsTissue EngineeringCollagen Type ICross-Linking ReagentsHydrogels
Identifiers
PMID40554673
PMCPMC12715433
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