Evidence map›Paper›PMID 40553284›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Unmasking Stemness and Immune Cell Markers in Brain Tumors with Immunohistochemistry.

Jack Q Lu, Kui Zhai, Agata M Kieliszek, Chitra Venugopal, Sheila K Singh

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Jack Q LuCentre for Discovery in Cancer Research, McMaster University, Hamilton, ON, Canada.
Kui ZhaiCentre for Discovery in Cancer Research, McMaster University, Hamilton, ON, Canada.
Agata M KieliszekCentre for Discovery in Cancer Research, McMaster University, Hamilton, ON, Canada.
Chitra VenugopalCentre for Discovery in Cancer Research, McMaster University, Hamilton, ON, Canada.
Sheila K SinghCentre for Discovery in Cancer Research, McMaster University, Hamilton, ON, Canada. ssingh@mcmaster.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Immunohistochemistry (IHC) is a useful method to detect and visualize targeted proteins in tissues, with a high degree of specificity and clarity. Through the strategic use of chromogens and counterstains, it enables a contrast-rich and accurate evaluation of specific molecules, such as surface markers. Here, we outline a comprehensive IHC followed with counterstaining protocol for the positive selection of stem-like cells and immune cells in brain tumor tissue.

Indexed as

Biomarkers, TumorBrain NeoplasmsImmunohistochemistryNeoplastic Stem CellsHumansStaining and LabelingBiomarkers, Tumor3,3′-DiaminobenzidineAntibodiesCD133CounterstainingHematoxylinIBA1Immunohistochemistry

Identifiers

PMID40553284

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.