Isolation and Culture of Human Neural Stem Cells.

Agata M Kieliszek, Yujin Suk, Daniel Mobilio, Chitra Venugopal, Sheila K Singh

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Agata M KieliszekDepartment of Surgery, McMaster University, Hamilton, ON, Canada.

Yujin SukCentre for Discovery in Cancer Research, McMaster University, Hamilton, ON, Canada.

Daniel MobilioCentre for Discovery in Cancer Research, McMaster University, Hamilton, ON, Canada.

Chitra VenugopalDepartment of Surgery, McMaster University, Hamilton, ON, Canada.

Sheila K SinghCentre for Discovery in Cancer Research, McMaster University, Hamilton, ON, Canada. ssingh@mcmaster.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A major hurdle in brain tumor therapy development is a lack of adequate controls to ensure therapeutic safety in human patients. Neural stem cells (NSCs) are healthy brain cells that are commonly used by neurobiologists and neuroscientists as controls for therapy development. Consequently, it is important that these cells are properly isolated and cultured to maintain cell integrity to efficiently uncover potential toxicities associated with developing therapies for downstream applications, including omics studies. In this chapter, we describe the optimized techniques for the isolation, acquisition, and maintenance of NSCs from fetal brain samples. For further details on the use of this protocol, please refer to Suk et al. (STAR Protoc 3:101628. https://doi.org/10.1016/J.XPRO.2022.101628 , 2022).

Indexed as

Cell Culture TechniquesCell SeparationNeural Stem CellsBrainCell DifferentiationCells, CulturedFetusHumansBrainCell cultureNeural stem cellsNeurosphereStem cell

Identifiers

PMID40553273

DOI10.1016/j.ccell.2022.10.009

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.