Evidence map›Paper›PMID 40553191›Full record

ArticlePlant cell reports2025

MeWRKY30, a cassava stress-responsive WRKY transcription factor, confers drought resistance to transgenic Arabidopsis.

Yufei Zhu, Zhibo Li, Wenjuan Wang, Xiaojie Liu, Qiuxian Xie, Xiaoling Yu, Xiuchun Zhang, Shuxia Li, Mengbin Ruan

Abstract read
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In one paragraph

Article in Plant cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Yufei ZhuNational Key Laboratory for Tropical Crop Breeding, Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.
Zhibo LiNational Key Laboratory for Tropical Crop Breeding, Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.
Wenjuan WangNational Key Laboratory for Tropical Crop Breeding, Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.
Xiaojie LiuNational Key Laboratory for Tropical Crop Breeding, Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.
Qiuxian XieNational Key Laboratory for Tropical Crop Breeding, Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.
Xiaoling YuNational Key Laboratory for Tropical Crop Breeding, Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.
Xiuchun ZhangNational Key Laboratory for Tropical Crop Breeding, Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.
Shuxia LiNational Key Laboratory for Tropical Crop Breeding, Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, China. lishuxia@itbb.org.cn.ORCID http://orcid.org/0000-0001-7329-9259
Mengbin RuanNational Key Laboratory for Tropical Crop Breeding, Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, China. ruanmenbin@itbb.org.cn.

Funding

Natural Science Foundation of China 32472190
6 · The paper itself

Abstract

key messageMeWRKY30 enhances drought tolerance by reducing ROS and MDA accumulation via the activation of stress-related transcription factors and ROS-scavenging genes. Cassava (Manihot esculenta Crantz), cultivated primarily in tropical regions, is susceptible to drought stress, which adversely affects its yield productivity. WRKY transcription factors play crucial roles in regulating drought responses; however, the specific molecular pathways in cassava remain to be characterized. Our previous transcriptomic analyses under drought stress identified MeWRKY30, the expression of which was upregulated by both drought and abscisic acid treatments. Comparative analysis revealed that Arabidopsis lines overexpressing MeWRKY30 exhibited enhanced drought tolerance, characterized by reduced malondialdehyde accumulation compared with wild-type controls, as well as delayed flowering and reproductive development. RNA sequencing (RNA-seq) identified 3,002 genes regulated by MeWRKY30, including AtNAC72, AtERF24, AtCAT2, and AtSPL9. Electrophoretic mobility shift assays (EMSAs) and dual-luciferase reporter assays confirmed that MeWRKY30 directly binds to W-box cis-elements in the AtERF24 promoter, thereby activating its transcription. These results suggest that MeWRKY30 is a positive regulator of drought tolerance in cassava and has potential applications in transgenic breeding programs.

Indexed as

ArabidopsisManihotPlant ProteinsTranscription FactorsDrought ResistanceDroughtsGene Expression Regulation, PlantPlants, Genetically ModifiedPromoter Regions, GeneticStress, PhysiologicalPlant ProteinsTranscription FactorsArabidopsisCassavaDrought toleranceMeWRKY30WRKY transcription factor

Identifiers

PMID40553191

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