Evidence map›Paper›PMID 40552939›Full record

ArticleAnalytical chemistry2025

Histone Analysis Using Mobility- and Mass-Selected Ultraviolet Photodissociation in Tandem with Ultra-High-Resolution Mass Spectrometry.

Cassandra N Fuller, Md Shofiul Alam, Kevin Jeanne Dit Fouque, Lilian Valadares Tose, Richard M Searfoss, Francisca N de Luna Vitorino, Mariangela Kosmopoulou, Detlev Suckau, Mark E Ridgeway, Steve Van Orden and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Linker H1 Histone Direct Top-Down Proteoform Analysis.Journal of proteome research · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Cassandra N FullerDepartment of Chemistry and Biochemistry, Florida International University, Miami, Florida 33199, United States.
Md Shofiul AlamDepartment of Chemistry and Biochemistry, Florida International University, Miami, Florida 33199, United States.
Kevin Jeanne Dit FouqueDepartment of Chemistry and Biochemistry, Florida International University, Miami, Florida 33199, United States.
Lilian Valadares ToseDepartment of Chemistry and Biochemistry, Florida International University, Miami, Florida 33199, United States.
Richard M SearfossDepartment of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri 63110, United States.
Francisca N de Luna VitorinoDepartment of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri 63110, United States.
Mariangela KosmopoulouFasmatech Science & Technology, Andrea Kalvou 5, Chalandri, 15232 Athens, Greece.
Detlev SuckauBruker Daltonics GmbH & Co. KG, 28359 Bremen, Germany.ORCID 0000-0002-1859-2492
Mark E RidgewayBruker Daltonics, Inc., Billerica, Massachusetts 01821, United States.
Steve Van OrdenBruker Daltonics, Inc., Billerica, Massachusetts 01821, United States.
Benjamin A GarciaDepartment of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri 63110, United States.ORCID 0000-0002-2306-1207
Francisco Fernandez-LimaDepartment of Chemistry and Biochemistry, Florida International University, Miami, Florida 33199, United States.ORCID 0000-0002-1283-4390

Funding

Development of biological structural tools for the study of protein-DNA complexesR35GM153450 · NIGMS · FLORIDA INTERNATIONAL UNIVERSITY · PI Francisco Fernandez-Lima · 2024 to 2026
$1.2M
NIGMS NIH HHS R35 GM153450
6 · The paper itself

Abstract

Ultra-high-resolution Fourier transform ion cyclotron resonance mass spectrometry (UHR FT-ICR MS) for top-down proteomics has shown the potential to resolve proteoforms and splice variants, particularly those with post-translational modifications. Here, we integrate trapped ion mobility and mass selection in tandem with ultraviolet photodissociation (UVPD) followed by FT-ICR MS measurements. The proposed method using mobility/mass-selected UVPD before FT-ICR MS allows for high protein sequence coverage and PTM localization with high mass accuracy (<1 ppm) and a better duty cycle (2×). When applied to the analysis of a bovine histone mixture, characteristic UVPD

Indexed as

HistonesUltraviolet RaysAnimalsCattleMass SpectrometryProtein Processing, Post-TranslationalProteomicsTandem Mass SpectrometryHistones

Identifiers

PMID40552939
PMCPMC12312367

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.