Axonemal Dynein Visualized in Primary Cilia via Expansion Microscopy.

Abstract read

In one paragraph

Article in Cytoskeleton (Hoboken, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

Update of
Ultrastructure expansion microscopy of axonemal dynein in islet primary cilia.2025

5 · Who and what money

Authors and funding

3 authors.

Xinhang DongDepartment of Biomedical Engineering, McKelvey School of Engineering, Washington University, Saint Louis, Missouri, USA.

Jeong Hun JoDepartment of Medicine, Division of Endocrinology, Metabolism, and Lipid Research, Washington University School of Medicine, Saint Louis, Missouri, USA.ORCID https://orcid.org/0000-0002-1056-0496

Jing HughesDepartment of Medicine, Division of Endocrinology, Metabolism, and Lipid Research, Washington University School of Medicine, Saint Louis, Missouri, USA.ORCID https://orcid.org/0000-0003-4397-8175

Funding

WU P&FP30DK020579 · NIDDK · WASHINGTON UNIVERSITY · PI Clay F. Semenkovich · 2013 to 2026

$27.1M

Metabolic signaling of the beta cell primary ciliumR01DK140365 · NIDDK · YALE UNIVERSITY · PI Jing Wang Hughes, Matthew J. Merrins · 2024 to 2026

$1.9M

Paracrine regulation of islet cell function by primary ciliaR01DK138974 · NIDDK · YALE UNIVERSITY · PI Jing Wang Hughes · 2024 to 2026

$1.3M

NIDDK NIH HHS 138974NIDDK NIH HHS 140365NIDDK NIH HHS P30 DK020579NIDDK NIH HHS R01 DK138974NIDDK NIH HHS R01 DK140365

6 · The paper itself

Abstract

Primary cilia are essential sensory organelles whose structural complexity has challenged detailed imaging analysis. Ultrastructure expansion microscopy (U-ExM) offers a promising approach by physically enlarging specimens in hydrogels, enabling nanoscale protein mapping. Here, we apply U-ExM to pancreatic islet cilia and demonstrate the conserved presence of all four axonemal dynein subtypes, including prominent localization of the intermediate chain DNAI1 in both primary cilia and centrioles. These findings suggest that U-ExM is a robust tool for ciliary studies and provide evidence that primary cilia may possess motor capabilities that could reshape our understanding of their function.

Indexed as

Axonemal DyneinsCiliaMicroscopyAnimalsCentriolesAxonemal Dyneinsaxonemal dyneinpancreatic isletsprimary ciliaultrastructure expansion microscopy

Identifiers

PMID40552592

DOI10.1002/cm.70004

PMCPMC12354234

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.