ArticleAnalytical chemistry2025
Multicolumn Two-Dimensional Liquid Chromatography Screening Platform for Stereopeptidomics and Application to Antimicrobial Peptide Polyene and Lipopeptide.
Article in Analytical chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
2 citing papers in PubMed.
- Integrated 1D-LC-HRMS and Heart-Cutting 2D-LC-MS for Impurity Profiling and Chiral Separation of Mitiglinide Drug Substance.Rapid communications in mass spectrometry : RCM · 2026Article
- Recent Developments in Capillary and Microchip Electroseparations of Peptides (2023-mid 2025).Electrophoresis · 2026Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Therapeutic peptides are a rapidly growing field in research and drug development. While the majority of natural and synthetic therapeutic peptides have l-amino acids as building blocks, d-amino acid-containing peptides are found frequently in nonribosomal peptides or can be formed during peptide synthesis by epimerization. Thus, analytical methods are needed for the quality control of stereointegrity and the determination of absolute configurations. Enantioselective amino acid analysis following complete hydrolysis is indispensable in the field but leads to the loss of sequence information, i.e., the position of d-amino acids can no longer be unambiguously assigned. Here, we propose a multicolumn two-dimensional liquid chromatography-tandem high-resolution mass spectrometry (2D LC-HRMS) platform with multiple reversed-phase type columns (C18, charged surface hybrid C18, mixed-mode C18 AX) in the first dimension (
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.