Evidence map›Paper›PMID 40551173›Full record

ArticleStem cell research & therapy2025

Mesenchymal stem cells and their secretome modulate stress and enhance lipogenesis in bovine mammary epithelial cells.

Roni Tadmor-Levi, Lior Sharabi, Adi Koren, Sharon Schlesinger, Noam Tzirkel-Hancock, Nurit Argov-Argaman

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Article in Stem cell research & therapy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Roni Tadmor-Levi *The Robert H. Smith Faculty of Agriculture, Food and Environment Sciences, The Hebrew University of Jerusalem, POB 12, 7610001, Rehovot, Israel.
Lior Sharabi *The Robert H. Smith Faculty of Agriculture, Food and Environment Sciences, The Hebrew University of Jerusalem, POB 12, 7610001, Rehovot, Israel.
Adi KorenThe Robert H. Smith Faculty of Agriculture, Food and Environment Sciences, The Hebrew University of Jerusalem, POB 12, 7610001, Rehovot, Israel.
Sharon SchlesingerThe Robert H. Smith Faculty of Agriculture, Food and Environment Sciences, The Hebrew University of Jerusalem, POB 12, 7610001, Rehovot, Israel.
Noam Tzirkel-HancockThe Robert H. Smith Faculty of Agriculture, Food and Environment Sciences, The Hebrew University of Jerusalem, POB 12, 7610001, Rehovot, Israel.
Nurit Argov-ArgamanThe Robert H. Smith Faculty of Agriculture, Food and Environment Sciences, The Hebrew University of Jerusalem, POB 12, 7610001, Rehovot, Israel. argov.nurit@mail.huji.ac.il.

Funding

Wilk technologies 3011005221
6 · The paper itself

Abstract

backgroundIncreased production of the modern dairy cow can induce a stress response by the mammary epithelial cells (MEC) and compromise production traits. To alleviate this stress response, various strategies have been tested. Previous studies have shown an immunomodulatory effect of mesenchymal stem cells (MSC) and their secretome on different cell types and tissues; however, their effect in the context of lactation performance has not yet been studied. In this study, we aimed to assess the effects of MSC and their secretome on the stress response and lipogenesis in bovine MEC.

methodsWe measured gene expression, lipid droplet (LD) characteristics, and triglyceride content in bovine MEC subjected to stress. The effect of co-culturing with MSC or their secretome, used as conditioned media (CM) was evaluated in the same context.

resultsIn MEC, lipopolysaccharide (LPS) triggered a progressive rise in pro-inflammatory cytokines, while H

conclusionsThese results demonstrate that the lipogenic capacity of MEC as well as their intracellular LD size and number, are integral to the stress response. MSC exerted an immunomodulatory effect on MEC and enhanced their lipogenic capacity. This effect is at least partly mediated by paracrine factors, and does not require physical contact between MEC and MSC. Further studies are warranted to identify the bioactive components, which could be used to enhance MEC bioactivity during lactation.

Indexed as

Epithelial CellsLipogenesisMammary Glands, AnimalMesenchymal Stem CellsSecretomeStress, PhysiologicalAnimalsCattleCoculture TechniquesCulture Media, ConditionedCytokinesFemaleHydrogen PeroxideLipopolysaccharidesTriglyceridesCulture Media, ConditionedCytokinesHydrogen PeroxideLipopolysaccharidesTriglyceridesConditioned mediaImmunomodulationLactationLipid dropletsMammary epithelial cellsMesenchymal stem cellsMilk fat

Identifiers

PMID40551173
PMCPMC12186326

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.