Evidence map›Paper›PMID 40550841›Full record

ArticleScientific reports2025

Cryogenic mouse tissue homogenization as an alternative to fresh-frozen biopsy use for genomics, transcriptomics, proteomics and metabolomics.

Laimdota Zizmare, Ute Hofmann, Mohamed Ali Jarboui, Franziska Klose, Sabine Fraschka, Jakob Matthes, Marcel Krüger, Elke Schaeffeler, Matthias Schwab, Marius Ueffing and 4 more

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Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
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  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Laimdota ZizmareWerner Siemens Imaging Center, Department of Preclinical Imaging and Radiopharmacy, University of Tübingen, 72076, Tübingen, Germany.
Ute HofmannDr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, University of Tübingen, 70376, Stuttgart, Germany.
Mohamed Ali JarbouiCore Facility for Medical Proteomics, Institute for Ophthalmic Research, University of Tübingen, 72076, Tübingen, Germany.
Franziska KloseCore Facility for Medical Proteomics, Institute for Ophthalmic Research, University of Tübingen, 72076, Tübingen, Germany.
Sabine FraschkaNGS Competence Center Tübingen (NCCT), Institute of Medical Genetics and Applied Genomics, University of Tübingen, 72076, Tübingen, Germany.
Jakob MatthesNGS Competence Center Tübingen (NCCT), Institute of Medical Genetics and Applied Genomics, University of Tübingen, 72076, Tübingen, Germany.
Marcel KrügerWerner Siemens Imaging Center, Department of Preclinical Imaging and Radiopharmacy, University of Tübingen, 72076, Tübingen, Germany.
Elke SchaeffelerCluster of Excellence iFIT (EXC2180) "Image-Guided and Functionally Instructed Tumor Therapies", University of Tübingen, 72076, Tübingen, Germany.
Matthias SchwabCluster of Excellence iFIT (EXC2180) "Image-Guided and Functionally Instructed Tumor Therapies", University of Tübingen, 72076, Tübingen, Germany.
Marius UeffingCore Facility for Medical Proteomics, Institute for Ophthalmic Research, University of Tübingen, 72076, Tübingen, Germany.
Bernd J PichlerWerner Siemens Imaging Center, Department of Preclinical Imaging and Radiopharmacy, University of Tübingen, 72076, Tübingen, Germany.
Karsten BoldtCore Facility for Medical Proteomics, Institute for Ophthalmic Research, University of Tübingen, 72076, Tübingen, Germany.
Nicolas CasadeiNGS Competence Center Tübingen (NCCT), Institute of Medical Genetics and Applied Genomics, University of Tübingen, 72076, Tübingen, Germany.
Christoph TrautweinWerner Siemens Imaging Center, Department of Preclinical Imaging and Radiopharmacy, University of Tübingen, 72076, Tübingen, Germany. Christoph.Trautwein@med.uni-tuebingen.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The classical approach of using adjacent pieces of fresh-frozen tissue for various omics analysis from the same sample possesses a risk of biological mismatch between arising from intrinsic tissue heterogeneity. We propose an alternative approach of tissue cryogenic pulverization and lyophilization before distribution for omics studies for a more reliable analysis. Here, we compare individual omics layer readouts from fresh-frozen adjacent tissue pieces and homogenized powder in mouse brain, kidney, and liver. Genomics, transcriptomics, proteomics, and metabolomics analyses showed comparable RNA integrity, DNA methylation, and coverage of transcripts, proteins, and metabolites across both methods. Moreover, the homogenized-lyophilized powder usage led to reduced heterogeneity between biological replicates. We conclude that the cryogenically pulverized-lyophilized tissue approach not only maintains a critical molecular feature coverage and quality but also provides a homogenous basis for various omics analysis enhancing reproducibility, sample transport, storage and enabling multi omics base on one and the same tissue aliquot.

Indexed as

CryopreservationGenomicsMetabolomicsProteomicsTranscriptomeAnimalsBiopsyBrainFreeze DryingGene Expression ProfilingKidneyLiverMice

Identifiers

PMID40550841
PMCPMC12185734

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.