Evidence map›Paper›PMID 40550785›Full record

ArticleACS applied materials & interfaces2025

Hydrogel Enhanced Organoid Multidirectional Differentiation via Yap/Tead4 Mechanotransduction for Accelerated Tissue Regeneration.

Peng Luo, Yuning Cheng, Yuwen Luo, Nan Zhang, Jingjing Cao, Honggang Wang, Xieyuan Jiang, Qian Wang, Xinbao Wu, Yajun Liu and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS applied materials & interfaces, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Peng LuoLaboratory of Bone Tissue Engineering, Beijing Laboratory of Biomedical Materials, National Center for Orthopaedics, Beijing Research Institute of Traumatology and Orthopaedics, Beijing Jishuitan Hospital, Capital Medical University, Beijing 100035, China.
Yuning ChengLaboratory of Bone Tissue Engineering, Beijing Laboratory of Biomedical Materials, National Center for Orthopaedics, Beijing Research Institute of Traumatology and Orthopaedics, Beijing Jishuitan Hospital, Capital Medical University, Beijing 100035, China.
Yuwen LuoLaboratory of Bone Tissue Engineering, Beijing Laboratory of Biomedical Materials, National Center for Orthopaedics, Beijing Research Institute of Traumatology and Orthopaedics, Beijing Jishuitan Hospital, Capital Medical University, Beijing 100035, China.
Nan ZhangLaboratory of Bone Tissue Engineering, Beijing Laboratory of Biomedical Materials, National Center for Orthopaedics, Beijing Research Institute of Traumatology and Orthopaedics, Beijing Jishuitan Hospital, Capital Medical University, Beijing 100035, China.
Jingjing CaoLaboratory of Bone Tissue Engineering, Beijing Laboratory of Biomedical Materials, National Center for Orthopaedics, Beijing Research Institute of Traumatology and Orthopaedics, Beijing Jishuitan Hospital, Capital Medical University, Beijing 100035, China.
Honggang WangLaboratory of Bone Tissue Engineering, Beijing Laboratory of Biomedical Materials, National Center for Orthopaedics, Beijing Research Institute of Traumatology and Orthopaedics, Beijing Jishuitan Hospital, Capital Medical University, Beijing 100035, China.
Xieyuan JiangDepartment of Orthopedic Trauma, National Center for Orthopaedics, Beijing Jishuitan Hospital, Capital Medical University, Beijing 100035, China.
Qian WangLaboratory of Bone Tissue Engineering, Beijing Laboratory of Biomedical Materials, National Center for Orthopaedics, Beijing Research Institute of Traumatology and Orthopaedics, Beijing Jishuitan Hospital, Capital Medical University, Beijing 100035, China.
Xinbao WuDepartment of Orthopedic Trauma, National Center for Orthopaedics, Beijing Jishuitan Hospital, Capital Medical University, Beijing 100035, China.
Yajun LiuDepartment of Spine Surgery, National Center for Orthopaedics, Beijing Jishuitan Hospital, Capital Medical University, Beijing 100035, China.
Jianping MaoDepartment of Spine Surgery, National Center for Orthopaedics, Beijing Jishuitan Hospital, Capital Medical University, Beijing 100035, China.
Xinhua ZhouDepartment of Orthopedic Surgery, National Center for Orthopaedics, Beijing Jishuitan Hospital, Capital Medical University, Beijing 100035, China.
Jing-Jun NieLaboratory of Bone Tissue Engineering, Beijing Laboratory of Biomedical Materials, National Center for Orthopaedics, Beijing Research Institute of Traumatology and Orthopaedics, Beijing Jishuitan Hospital, Capital Medical University, Beijing 100035, China.
Dafu ChenLaboratory of Bone Tissue Engineering, Beijing Laboratory of Biomedical Materials, National Center for Orthopaedics, Beijing Research Institute of Traumatology and Orthopaedics, Beijing Jishuitan Hospital, Capital Medical University, Beijing 100035, China.ORCID 0000-0002-1879-8239

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The repair of multiple organs in motor systems remains a major clinical challenge that necessitates bioactive grafts with a multidirectional differentiation ability. Hydrogel-based organoids implants have emerged as pivotal tools and attracted great attentions. However, strategies to unlock the multipotency of bone marrow mesenchymal stem cells (BMSCs) by precisely modulating the mechanical and structural characteristics of biomimetic extracellular matrix (ECM) during hydrogel-based organoid construction remain underexplored. In this study, a gelatin methacryloyl (GelMA)-based biomimetic ECM mimic hydrogel (HG-2) loaded with BMSCs was developed to construct a multidirectional differentiation organoid, HG-2/3d-BMSC. The hydrogel could provide spatial mechanical stimulation to adherent BMSCs via cell adhesion induced cytoskeleton assembly. RNA sequencing (RNA-Seq) combined with in vitro and in vivo biological experiments reveals that ECM mimic hydrogels deliver adhesion-based spatial mechanical stimulation. This mechanical stimulation specifically unlocks the multipotency of BMSCs during osteogenic differentiation induction. Furthermore, it accelerates and enhances the multidirectional differentiation capacity of BMSCs, simultaneously promoting their commitment to osteogenic, chondrogenic, and tendonogenic tissue lineages. Further investigations prove that adhesion-based spatial mechanical stimulation from the ECM mimic hydrogel enhances multidirectional differentiation of BMSCs-based organoid via Yap/Tead4 (yes-associated protein/TEA domain transcription factor 4) mechanotransduction mediated Kat7 downregulation. The work not only advances the theoretical framework for designing biomaterials that exploit mechanical cues to override biochemical-driven lineage commitment but also establishes a novel paradigm for developing multifunctional organoid constructs to address the clinical challenge of regenerating hierarchically complex tissues in a motor system.

Indexed as

DNA-Binding ProteinsHydrogelsMechanotransduction, CellularOrganoidsRegenerationTranscription FactorsAnimalsCell DifferentiationCells, CulturedExtracellular MatrixGelatinMesenchymal Stem CellsMethacrylatesMiceOsteogenesisRatsDNA-Binding ProteinsGelatingelatin methacryloylHydrogelsMethacrylatesTranscription FactorsYAP-Signaling ProteinsECM mimic hydrogelMultidirectional differentiationMultiple organs repairOrganoidYap/Tead4 mechanotransduction

Identifiers

PMID40550785
PMCPMC12226991

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.