Evidence map›Paper›PMID 40549708›Full record

ArticlePloS one2025

The bovine oviductal environment and composition are negatively affected by elevated body energy reserves.

Natália Marins Bastos, Rodrigo Silva Goulart, Alessandra Bridi, Rosane Mazzarella, Luana Alves, Paola Maria da Silva Rosa, Ricardo de Francisco Strefezzi, Lindsay Baltel Paskoski, Ricardo Perecin Nociti, Juliano Rodrigues Sangalli and 8 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Natália Marins BastosDepartment of Veterinary Medicine, College of Animal Science and Food Engineering, University of São Paulo, São Paulo, Brazil.
Rodrigo Silva GoulartDepartment of Animal Science, College of Animal Science and Food Engineering, University of São Paulo, São Paulo, Brazil.
Alessandra BridiDepartment of Veterinary Medicine, College of Animal Science and Food Engineering, University of São Paulo, São Paulo, Brazil.
Rosane MazzarellaDepartment of Veterinary Medicine, College of Animal Science and Food Engineering, University of São Paulo, São Paulo, Brazil.
Luana AlvesDepartment of Veterinary Medicine, College of Animal Science and Food Engineering, University of São Paulo, São Paulo, Brazil.
Paola Maria da Silva RosaDepartment of Veterinary Medicine, College of Animal Science and Food Engineering, University of São Paulo, São Paulo, Brazil.
Ricardo de Francisco StrefezziDepartment of Veterinary Medicine, College of Animal Science and Food Engineering, University of São Paulo, São Paulo, Brazil.ORCID 0000-0002-8810-2815
Lindsay Baltel PaskoskiDepartment of Veterinary Medicine, College of Animal Science and Food Engineering, University of São Paulo, São Paulo, Brazil.
Ricardo Perecin NocitiDepartment of Veterinary Medicine, College of Animal Science and Food Engineering, University of São Paulo, São Paulo, Brazil.
Juliano Rodrigues SangalliDepartment of Veterinary Medicine, College of Animal Science and Food Engineering, University of São Paulo, São Paulo, Brazil.
Schaienni Fontoura SaldanhaDepartment of Veterinary Medicine, College of Animal Science and Food Engineering, University of São Paulo, São Paulo, Brazil.
Camila Azzolin de SouzaDepartment of Veterinary Medicine, College of Animal Science and Food Engineering, University of São Paulo, São Paulo, Brazil.
Angélica Camargo Dos SantosDepartment of Genetics and Evolution, Federal University of São Carlos, São Carlos, São Paulo, Brazil.
Marcos Roberto ChiarattiDepartment of Genetics and Evolution, Federal University of São Carlos, São Carlos, São Paulo, Brazil.
Guilherme PugliesiDepartment of Animal Reproduction, Faculty of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.
Flávio Vieira MeirellesDepartment of Veterinary Medicine, College of Animal Science and Food Engineering, University of São Paulo, São Paulo, Brazil.
Felipe PerecinDepartment of Veterinary Medicine, College of Animal Science and Food Engineering, University of São Paulo, São Paulo, Brazil.
Juliano Coelho da SilveiraDepartment of Veterinary Medicine, College of Animal Science and Food Engineering, University of São Paulo, São Paulo, Brazil.ORCID 0000-0002-4796-6393

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

To analyze the effects of high body energy reserve (BER) within the oviductal environment and its composition, Nellore cows were fed two different nutritional plans to obtain animals with moderate BER (MBER) and high BER (HBER). After obtaining the groups with different BERs, all animals were subjected to oestrus synchronization and artificial insemination, and 120 hours after ovulation induction, the cows were slaughtered, the reproductive tract was removed, and the ipsilateral oviduct to the corpus luteum was collected and dissected. Analyses were performed only for animals that had an 8-cell embryo in the isthmus. After embryo identification, we evaluated the molecular profiles of extracellular vesicles from oviductal flushing (OF-EVs) and luminal epithelial cells (OV-Cell) and performed histomorphological analysis of oviductal tissue from the ampullary and isthmic oviductal regions. The HBER group presented higher concentrations of ampullary extracellular vesicles (AMP-EVs) and larger sizers of isthmic extracellular vesicles (IST-EVs). The miRNA profile of AMP-EVs showed that the differentially expressed miRNAs were predicted to regulate pathways associated with cell growth, migration, differentiation and metabolism, with the HBER group being more susceptible to insulin modulation. The MBER animals showed greater ampullary vascularization than the HBER animals did. Additionally, the miRNA profile and differential gene expression (DEG) data obtained for ampullary (AMP-Cell) and isthmic (IST-Cell) luminal epithelial cells revealed pathways related to insulin metabolism. Thus, elevated BER may lead to oviductal insulin resistance, affecting normal functioning and, probably, embryo metabolism during early development, thus impacting gestational rates in these animals.

Indexed as

Energy MetabolismFallopian TubesOviductsAnimalsCattleEpithelial CellsExtracellular VesiclesFemaleMicroRNAsMicroRNAs

Identifiers

PMID40549708
PMCPMC12184905

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.