Evidence map›Paper›PMID 40549002›Full record

ArticleCellular and molecular life sciences : CMLS2025

E-cadherin negatively regulates hESCs endodermal differentiation under varied substrate stiffnesses.

Fan Zhang, Lu Zheng, Yi Wu, Qihan Ding, Shouqin Lü, Dongyuan Lü, Mian Long

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular and molecular life sciences : CMLS, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Fan Zhang *Center for Biomechanics and Bioengineering, Beijing Key Laboratory of Engineered Construction and Mechanobiology and Key Laboratory of Microgravity, Institute of Mechanics, Chinese Academy of Sciences, Beijing, 100190, China.
Lu Zheng *Center for Biomechanics and Bioengineering, Beijing Key Laboratory of Engineered Construction and Mechanobiology and Key Laboratory of Microgravity, Institute of Mechanics, Chinese Academy of Sciences, Beijing, 100190, China.
Yi WuCenter for Biomechanics and Bioengineering, Beijing Key Laboratory of Engineered Construction and Mechanobiology and Key Laboratory of Microgravity, Institute of Mechanics, Chinese Academy of Sciences, Beijing, 100190, China.
Qihan DingCenter for Biomechanics and Bioengineering, Beijing Key Laboratory of Engineered Construction and Mechanobiology and Key Laboratory of Microgravity, Institute of Mechanics, Chinese Academy of Sciences, Beijing, 100190, China.
Shouqin LüCenter for Biomechanics and Bioengineering, Beijing Key Laboratory of Engineered Construction and Mechanobiology and Key Laboratory of Microgravity, Institute of Mechanics, Chinese Academy of Sciences, Beijing, 100190, China.
Dongyuan LüCenter for Biomechanics and Bioengineering, Beijing Key Laboratory of Engineered Construction and Mechanobiology and Key Laboratory of Microgravity, Institute of Mechanics, Chinese Academy of Sciences, Beijing, 100190, China. lvdongy@imech.ac.cn.
Mian LongCenter for Biomechanics and Bioengineering, Beijing Key Laboratory of Engineered Construction and Mechanobiology and Key Laboratory of Microgravity, Institute of Mechanics, Chinese Academy of Sciences, Beijing, 100190, China. mlong@imech.ac.cn.ORCID http://orcid.org/0000-0003-0819-6186

Funding

National Key Research and Development Program of China grant 2021YFA0719302National Natural Science Foundation of China grants 32130061National Natural Science Foundation of China grants 32371376
6 · The paper itself

Abstract

Intercellular adhesion is crucial in regulating stemness maintenance and differentiation initiation of embryonic stem cells (ESCs), which is also cooperated with extracellular mechanical microenvironment. Here an in vitro model was used to elucidate the effects of E-cadherin complexes on definitive endoderm (DE)-directed differentiation of hESCs (H1 cells), when the cells were seeded on polyacrylamide hydrogels with varied stiffnesses. Results indicated that stiff substrate increased the proportion of H1 cells differentiating into DE cells and intercellular E-cadherin expression was reduced with progressive stages at same stiffness, presenting a negative correlation of E-cadherin expression with differentiating progress or substrate stiffness. Blocking E-cadherin enhanced the productivity of differentiated cells and promoted the disassembly of intercellular adhesions by translocating YAP into nuclei, which was positively correlated with GATA6 and CXCR4 expressions in a stiffness-dependent manner. This work provided an insight into understanding the roles of E-cadherin-related intercellular adhesion and substrate stiffness in DE-directed differentiation of hESCs.

Indexed as

CadherinsCell DifferentiationEndodermHuman Embryonic Stem CellsAcrylic ResinsAntigens, CDCell AdhesionGATA6 Transcription FactorHumansHydrogelsPolyacrylamidesReceptors, CXCR4Transcription FactorsYAP-Signaling ProteinsAcrylic ResinsAntigens, CDCadherinsCDH1 protein, humanCXCR4 protein, humanGATA6 Transcription FactorHydrogelsPolyacrylamidesReceptors, CXCR4Transcription FactorsYAP1 protein, humanYAP-Signaling ProteinsDefinitive endodermal differentiationE-cadherinEmbryonic stem cellsSubstrate stiffnessYAP

Identifiers

PMID40549002
PMCPMC12185850

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.