ArticleJournal of proteome research2025
A Comparative Proteomics Study of Autopsy and Fresh-Frozen Coronary Artery Samples.
Article in Journal of proteome research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Aortic Stenosis Hyalectan Remodeling Revealed by Proteomics and Glycoproteomics.Arteriosclerosis, thrombosis, and vascular biology · 2026Article
- Consensus statement on mass spectrometry-based proteomic analysis of cardiac tissue.Nature cardiovascular research · 2026Article
- Impact of Variations in Commercial Standard Operating Procedure on Plasma Proteome Recovery.Journal of proteome research · 2026Article
- Fibroblast-like cells accumulate late in human coronary atherosclerosis contributing to necrotic core formation.Cardiovascular research · 2026Article
- Quantitative proteomics of formalin-fixed, paraffin-embedded cardiac specimens uncovers protein signatures of specialized regions and patient groups.Nature cardiovascular research · 2025Article
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Authors and funding
14 authors.
Funding
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Abstract
Proteomic analyses of human tissues are often conducted on autopsy samples. However, no detailed comparative analysis between proteomic changes derived from autopsy samples and fresh-frozen samples has been undertaken. In this study, human left anterior descending (LAD) coronary artery samples (n = 94) from deceased patients were analyzed using nanoflow LC-MS/MS. Among consistently quantified proteins, 37% of the protein abundances exhibited significant correlations with the post-mortem interval (PMI), most of which are inverse. Notably, smooth muscle cell markers displayed substantial reduction with prolonged PMI. Conversely, positive correlations were observed for immunoglobulins, coagulation factors, and complement factors, including coagulation factor XII, plasminogen, and lactotransferrin. Comparative analyses of sex-specific protein changes in autopsy LAD samples versus fresh-frozen LAD samples (n = 65) showed no concordance. However, a robust correlation was observed between 2 different cohorts of fresh-frozen carotid endarterectomies (n = 120 and n = 200). This study represents the first large-scale proteomics investigation into the influence of PMI on the protein composition of the human vasculature, showing significant correlations with PMI for 37% of the quantified proteins. Our findings underscore potential discrepancies in the quantitative accuracy of proteomics data derived from autopsy samples. Consequently, results obtained from post-mortem specimens may not be reproducible in fresh-frozen samples.
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