ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2025
Hyperspectral Fluorescence Imaging with a New Polarity-Ultrasensitive Fluorescent Probe.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
5 citing papers in PubMed.
- A dual-responsive fluorescent probe for imaging viscosity and polarity in nonalcoholic fatty liver disease.Smart molecules : open access · 2026Article
- Janus porphyrin-stabilized phase-transition theranostics for multimodal imaging-guided synergistic cancer therapy.Materials today. Bio · 2026Article
- 3D Single-Virus Tracking: Advances in Methodology and Labeling Strategies Towards Probing the Virus-Epithelium Interaction.Viruses · 2026Review
- Cell wash-free fluorescent probes based on phenothiazine and phenoxazine with high photostability and large stokes shifts for targeted imaging of subcellular organelles.Materials today. Bio · 2025Article
- Hyperspectral Fluorescence Imaging with a New Polarity-Ultrasensitive Fluorescent Probe.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Hyperspectral fluorescence imaging (HSFI) could simultaneously offer morphological visualization and microenvironmental information through fluorescence wavelength-shifting of microenvironmental-sensitive fluorescent probes, yet its advancement is directly hindered by the scarcity of probes with sufficient spectral sensitivity. Herein, this study focuses on the development of an innovative fluorescent probe that exhibits heightened spectral sensitivity to microenvironmental changes and its advanced HSFI application. First, the comprehensive investigation of various donor-π-acceptor molecules uncovers the critical role of π-spacer on the wavelength sensitivity to microenvironment polarity. Based on this insight, a new fluorescent probe Lipi-PS of whose wavelength sensitivity represents the highest level so far is rationally developed. Further combining with the features of lipid droplets (LDs) targeting and high photostability of Lipi-PS, as well as the newly established wavelength and polarity encoding method, HSFI is successfully realized in cells, tissues, and zebrafishes. Accordingly, the structural morphologies, fluorescence wavelengths, and polarities are intuitively visualized in the HSFI images. Notably, a special HSFI of single LD is achieved using Lipi-PS in a custom-built 3D-SpecDIM system, allowing 5D tracking (xyzλt) of individual LDs for the first time. This enables unprecedented correlation of polarity-dynamic behavior at the single-LD level.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.