Evidence map›Paper›PMID 40548435›Full record

ArticleJournal of microscopy2025

Virtual dissection of Aedes aegypti mosquito using phase-contrast synchrotron microtomography.

Gabriela Sena, Thaina Alvarenga, Ruan Ingliton Feio, Cícero Brasileiro Mello, Gabriel Fidalgo, Katrine Paiva, Tayane Tanure, Liebert Parreiras Nogueira, Marcos Vinícius Colaço, Arissa Pickler and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of microscopy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Gabriela SenaLaboratory of Applied Physics to Biomedical Sciences, Physics Institute, State University of Rio de Janeiro, Rio de Janeiro, Brazil.ORCID 0000-0003-1369-7460
Thaina AlvarengaLaboratory of Applied Physics to Biomedical Sciences, Physics Institute, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
Ruan Ingliton FeioPost-Graduate Program in Science and Biotechnology, Federal University Fluminense, Rio de Janeiro, Brazil.
Cícero Brasileiro MelloPost-Graduate Program in Science and Biotechnology, Federal University Fluminense, Rio de Janeiro, Brazil.
Gabriel FidalgoLaboratory of Applied Physics to Biomedical Sciences, Physics Institute, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
Katrine PaivaLaboratory of Applied Physics to Biomedical Sciences, Physics Institute, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
Tayane TanureLaboratory of Applied Physics to Biomedical Sciences, Physics Institute, State University of Rio de Janeiro, Rio de Janeiro, Brazil.ORCID 0000-0002-4442-9920
Liebert Parreiras NogueiraOral Research Laboratory (ORL), Institute of Clinical Dentistry, University of Oslo, Oslo, Norway.ORCID 0000-0002-7767-464X
Marcos Vinícius ColaçoLaboratory of Applied Physics to Biomedical Sciences, Physics Institute, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
Arissa PicklerLaboratory of Applied Physics to Biomedical Sciences, Physics Institute, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
Marcelo Salabert GonzalezPost-Graduate Program in Science and Biotechnology, Federal University Fluminense, Rio de Janeiro, Brazil.
Patricia AzambujaPost-Graduate Program in Science and Biotechnology, Federal University Fluminense, Rio de Janeiro, Brazil.
Giuliana TrombaSYRMEP, Elettra-Sincrotrone Trieste SCpA, Trieste, Italy.
José Bento Pereira LimaLaboratory of Biology, Control and Surveillance of Insect Vectors, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil.
Ademir Xavier da SilvaNuclear Engineering Department, COPPE, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
Regina Cély BarrosoLaboratory of Applied Physics to Biomedical Sciences, Physics Institute, State University of Rio de Janeiro, Rio de Janeiro, Brazil.

Funding

CAPESCNPqFAPERJ
6 · The paper itself

Abstract

In this paper, in-line phase-contrast synchrotron microtomography was used to study the morphology of adult Aedes aegypti. These specimens are vectors of several arboviruses, causing dengue, chikungunya, Zika and yellow fever. The morphological details of this insect species are still incomplete and insufficient. To address this gap, this study examined whole specimens of Aedes aegypti in the adult phase at high resolution. For this, the adult samples were scanned in the microtomography beamline (SYRMEP) at the Italian Synchrotron Light Laboratory (ELETTRA). The phase-contrast technique allowed us to obtain high-quality images, which made it possible to evaluate the segmentation of structures on the rendered volume by the Dragonfly software. The combination of high-quality images and segmentation process provide adequate visualisation of different organs which could serve in assessing the effectiveness of innovative control population methods as a basis for future control studies of the insect vector.

Indexed as

AedesMosquito VectorsX-Ray MicrotomographyAnimalsFemaleImage Processing, Computer-AssistedMicroscopy, Phase-ContrastSynchrotronsaedes aegyptimicrotomographyphase‐contrast

Identifiers

PMID40548435
PMCPMC12459427

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.