ArticleRegenerative therapy2025
Development of pyrogen-free utensils for cell culture by combining ozone/hydrogen peroxide mixed gas sterilization with steam-washing technology.
Article in Regenerative therapy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Study on the relationship between bacterial endotoxin and the onset of toxic anterior segment syndrome.Journal of cataract and refractive surgery · 2026Article
- Quality assessment of endotoxin contamination in consumables used for assisted reproductive technology.PloS one · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Objective: The aim of this study was to develop disposable cell culture utensils with improved cleanliness. Methods: Pyrogen contamination levels were evaluated using a direct human cell-based pyrogen test (HCPT). Pyrogen inactivation tests were performed using low-temperature ozone/hydrogen peroxide mixed gas treatment. Residual hydrogen peroxide was quantified using a commercially available measurement kit, and the effectiveness of vacuum drying and steam washing in removing the residual hydrogen peroxide was evaluated. The physicochemical properties of the materials were analyzed by measuring FI-IR spectra, static contact angles, and tensile strength. Results: The HCPT of commercially available pyrogen-free products showed that two out of eight pipette chips were contaminated with pyrogen exceeding the limit of quantitation. For the well plates and conical tubes, two out of eleven and four out of ten of the products, respectively, were identified as pyrogen-positive. Some products were contaminated with trace amounts of pyrogen. Pyrogens present in these products were inactivated to below the limit of detection by ozone/hydrogen peroxide mixed gas treatment at 50 °C. Residual hydrogen peroxide on the surface of the products after the treatment, that exhibits strong oxidative effects to cultured cells, was removed to almost the limit of detection by sequential vacuum drying (80 °C/24 h) and steam-washing at 50 °C. Polypropylene and polystyrene, as raw materials for the products, were physicochemically stable for mixed gas treatment. Conclusion: These results suggest that the production of cell culture utensils containing no pyrogen and with no influence on the cell culture is now possible by installing these technologies in the manufacturing process, instead of the radiation sterilization currently used.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.