Evidence map›Paper›PMID 40547232›Full record

ArticleMetabarcoding and metagenomics2025

What is eDNA method standardisation and why do we need it?

Susanna Theroux, Adam Sepulveda, Cathryn L Abbott, Zachary Gold, Alison W Watts, Margaret E Hunter, Katy E Klymus, Shana Lee Hirsch, Joseph M Craine, Devin N Jones and 5 more

Abstract read
In one paragraph

Article in Metabarcoding and metagenomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
  4. Fungal ecology in the age of 'omics.The New phytologist · 2026
    Review
  5. Article
  6. Review
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Susanna TherouxSouthern California Coastal Water Research Project, Costa Mesa, CA, USA.ORCID 0000-0002-9812-7856
Adam SepulvedaU.S. Geological Survey, Northern Rocky Mountain Science Center, Bozeman, MT, USA.ORCID 0000-0001-7621-7028
Cathryn L AbbottFisheries and Oceans Canada, Nanaimo, British Columbia, Canada.ORCID 0000-0002-5314-7351
Zachary GoldNOAA Pacific Marine Environmental Laboratory, Seattle, WA, USA.ORCID 0000-0003-0490-7630
Alison W WattsUniversity of New Hampshire, Durham, NH, USA.ORCID 0000-0001-9700-6393
Margaret E HunterU.S. Geological Survey, Wetland and Aquatic Research Center, Gainesville, FL, USA.
Katy E KlymusU.S. Geological Survey, Columbia Environmental Research Center, Columbia, OH, USA.ORCID 0000-0002-8843-6241
Shana Lee HirschUniversity of Washington, Seattle, WA, USA.ORCID 0000-0003-3131-1701
Joseph M CraineJonah Ventures, Boulder, CO, USA.
Devin N JonesU.S. Geological Survey, Northern Rocky Mountain Science Center, Bozeman, MT, USA.ORCID 0000-0001-9215-2930
Rachel J BrownWhitney Genetics Laboratory, US Fish and Wildlife Service, Onalaska, WI, USA.ORCID 0000-0001-5353-715X
Joshua A SteeleSouthern California Coastal Water Research Project, Costa Mesa, CA, USA.
Miwa TakahashiCommonwealth Scientific and Industrial Research Organisation, Crawley, Australia.ORCID 0000-0001-8952-051X
Rachel T NobleInstitute of Marine Sciences, UNC Chapel Hill, Morehead City, NC, USA.
John A DarlingCenter for Ecological Measurement & Modeling, US Environmental Protection Agency, Research Triangle Park, NC, USA.

Funding

Intramural EPA EPA999999
6 · The paper itself

Abstract

The rapid advancement of environmental DNA (eDNA) science in the past two decades has inspired a concomitant growth in the development of eDNA sampling and analytical methods. However, these methods are often developed by individual laboratories or institutions, which can isolate protocols within programmes, agencies or regions and prevent the beneficial exchange of data and ideas. Recent efforts to advance national and international coordination have resulted in a groundswell of standardisation efforts, but there is still considerable confusion around the role of formal standards for regulatory or research applications. With this commentary, we hope to provide clarity on the terminology used in standardisation discussions, including the differences between formal standards and best practice guidelines. Additionally, we discuss how eDNA method choice may be informed by environmental management scenarios and review examples of formal eDNA method standards being used to inform management action. The eDNA community now has an opportunity to develop a roadmap for method development to help close standardisation gaps, advance eDNA method adoption and accelerate our ability to monitor biological life at the scales our current environmental challenges demand.

Indexed as

Environmental DNAlab accreditationmanagementmethodsstandardisation

Identifiers

PMID40547232
PMCPMC12180942

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.