Evidence map›Paper›PMID 40546952›Full record

ArticleiScience2025

Regulation of Rho guanine nucleotide exchange factor 3 by phosphorylation in the PH domain.

Jesus F Moreno, Jae-Sung You, Carlos C Rodriguez, Shashank Pant, Adriana Reyes-Ordoñez, Reean Abdullah, Nilmani Singh, Maxine J van der Donk, Emad Tajkhorshid, Jie Chen

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In one paragraph

Article in iScience, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Jesus F MorenoDepartment of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
Jae-Sung YouDepartment of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
Carlos C RodriguezDepartment of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
Shashank PantDepartment of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
Adriana Reyes-OrdoñezDepartment of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
Reean AbdullahDepartment of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
Nilmani SinghDepartment of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
Maxine J van der DonkDepartment of Chemistry, Northwestern University, Evanston, IL, USA.
Emad TajkhorshidDepartment of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
Jie ChenDepartment of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Although most members of the Rho guanine nucleotide exchange factor (RhoGEF) family are found to be phosphorylated, how phosphorylation regulates RhoGEF function is poorly understood. Here we report the discovery of a mechanism of RhoGEF regulation by phosphorylation. We find that ARHGEF3 is phosphorylated in the pleckstrin homology (PH) domain in a protein kinase C (PKC)-dependent manner. This phosphorylation inhibits ARHGEF3 activation of RhoA and actin stress fiber formation in the cells, and it also disrupts ARHGEF3 binding to PI(3,5)P

Indexed as

BiochemistryIn silico biologyMolecular biology

Identifiers

PMID40546952
PMCPMC12178798

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.