Evidence map›Paper›PMID 40546673›Full record

ArticleWorld journal of experimental medicine2025

Platelet rich plasma, adipose tissue micrografts, and regenerative mimetic factors for abdominal wall defect reconstruction: Experimental study protocol.

Konstantinos Zapsalis, Orestis Ioannidis, Christos Xylas, Konstantinos Siozos, Georgios Gemousakakis, Elissavet Anestiadou, Savvas Symeonidis, Stefanos Bitsianis, Efstathios Kotidis, Angeliki Cheva and 8 more

Abstract read
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In one paragraph

Article in World journal of experimental medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Konstantinos ZapsalisFourth Department of Surgery, Medical School, Aristotle University of Thessaloniki, Thessaloniki 57010, Kentrikí Makedonía, Greece.
Orestis IoannidisFourth Department of Surgery, Medical School, Aristotle University of Thessaloniki, Thessaloniki 57010, Kentrikí Makedonía, Greece.
Christos XylasFourth Department of Surgery, Medical School, Aristotle University of Thessaloniki, Thessaloniki 57010, Kentrikí Makedonía, Greece.
Konstantinos SiozosFourth Department of Surgery, Medical School, Aristotle University of Thessaloniki, Thessaloniki 57010, Kentrikí Makedonía, Greece.
Georgios GemousakakisFourth Department of Surgery, Medical School, Aristotle University of Thessaloniki, Thessaloniki 57010, Kentrikí Makedonía, Greece.
Elissavet AnestiadouFourth Department of Surgery, Medical School, Aristotle University of Thessaloniki, Thessaloniki 57010, Kentrikí Makedonía, Greece.
Savvas SymeonidisFourth Department of Surgery, Medical School, Aristotle University of Thessaloniki, Thessaloniki 57010, Kentrikí Makedonía, Greece.
Stefanos BitsianisFourth Department of Surgery, Medical School, Aristotle University of Thessaloniki, Thessaloniki 57010, Kentrikí Makedonía, Greece.
Efstathios KotidisFourth Department of Surgery, Medical School, Aristotle University of Thessaloniki, Thessaloniki 57010, Kentrikí Makedonía, Greece.
Angeliki ChevaDepartment of Pathology, Medical School, Aristotle University of Thessaloniki, Thessaloniki 54636, Kentrikí Makedonía, Greece.
Chryssa BekiariLaboratory of Anatomy and Histology, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki 54124, Kentrikí Makedonía, Greece.
Antonia LoukousiaDepartment of Pathology, Medical School, Aristotle University of Thessaloniki, Thessaloniki 54636, Kentrikí Makedonía, Greece.
Konstantinos AngelopoulosFourth Department of Surgery, Medical School, Aristotle University of Thessaloniki, Thessaloniki 57010, Kentrikí Makedonía, Greece. konstzapsalis@yahoo.gr.
Manousos-Georgios PramateftakisFourth Department of Surgery, Medical School, Aristotle University of Thessaloniki, Thessaloniki 57010, Kentrikí Makedonía, Greece.
Ioannis MantzorosFourth Department of Surgery, Medical School, Aristotle University of Thessaloniki, Thessaloniki 57010, Kentrikí Makedonía, Greece.
Freiderikos TserkezidisFourth Department of Surgery, Medical School, Aristotle University of Thessaloniki, Thessaloniki 57010, Kentrikí Makedonía, Greece.
Barbara DriagkaFourth Department of Surgery, Medical School, Aristotle University of Thessaloniki, Thessaloniki 57010, Kentrikí Makedonía, Greece.
Stamatios AngelopoulosFourth Department of Surgery, Medical School, Aristotle University of Thessaloniki, Thessaloniki 57010, Kentrikí Makedonía, Greece.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundIncisional hernias are a common complication of previous surgeries and remain a persistent issue in clinical practice, posing a significant burden on healthcare systems despite advances in education and technology. Surgical techniques, primarily involving the use of mesh to cover the abdominal wall gap, are widely used as a standard intervention strategy.

aimTo examine the regeneration of the aponeurosis defect in the anterior abdominal wall in rats using regenerative mimetic factors of the extracellular matrix [ReGeneraTing Agent (RGTA)], adipose tissue micrografts (ATM), and platelet rich plasma (PRP) as regenerative agents.

methodsRegenerative agents such as RGTA, ATM, and PRP are gaining popularity. ATM involves autologous adipose tissue cells with mesenchymal stem cell markers and a high percentage of stromal vascular fraction cells. RGTAs are heparan sulfate (HS) mimetics that replace degraded HSs in damaged tissue, enhancing the quality and speed of repair. PRP is a concentrated plasma preparation containing seven fundamental proteins responsible for tissue production. An acellular dermal matrix is a biological implant free of cellular or antigenic components, making it an excellent material for reconstructive surgery. Polyglactin is a synthetic, absorbable mesh that loses 50% of its strength after fourteen days, providing initial support for new tissue regeneration before being completely absorbed.

resultsRats will undergo a laparotomy with a precise 2 cm by 2 cm excision of the anterior abdominal wall fascia below the umbilicus. They will be divided into sixteen groups, each receiving different combinations of regenerative factor injections into the denervated area in both non-contaminated and contaminated environments. A collagen-elastin matrix will be used to join the aponeurosis edges, with an absorbable polyglactin mesh anchored over it. Samples will be taken for macroscopic, histological, and immunohistochemical evaluation of tissue regeneration.

conclusionOur study aims to demonstrate how these factors promote cell proliferation and healing of the denervated anterior abdominal wall, potentially reducing the frequency and complications of incisional hernias. This approach could offer a more economical and efficient treatment option compared to current costly methods.

Indexed as

Abdominal wall defectsAcellular collagen–elastin matrixAdipose tissue micrograftsIncisional herniaLaparotomyPlatelet rich plasmaPolyglactin meshRegenerative agentsRegenerative mimetic factors

Identifiers

PMID40546673
PMCPMC12019615

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.