Evidence map›Paper›PMID 40545972›Full record

ArticleJournal of extracellular vesicles2025

Metabolically Engineered Extracellular Vesicles Released From a Composite Hydrogel Delivery System Regulate the Microenvironment for Periprosthetic Osteolysis Treatment.

Chenchen Wang, Jiang Ju, Chao Fu, Bingbo Bao, Tianhui Ren, Yanan Li, Yuan Wang, Sheng Han, Yuan Wang, Xuan Huang and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of extracellular vesicles, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
  4. Clinical Applications of Extracellular Vesicles: Promises and Pitfalls.International journal of molecular sciences · 2026
    Review
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Chenchen WangDepartment of Orthopedic Surgery, Shanghai Sixth People's Hospital Affiliated to Shanghai JiaoTong University School of Medicine, Shanghai, China.
Jiang JuSchool of Chemistry and Chemical Engineering, Key Laboratory of Thin Film and Microfabrication Technology (Ministry of Education), Shanghai Jiao Tong University, Shanghai, China.
Chao FuSchool of Chemistry and Chemical Engineering, Key Laboratory of Thin Film and Microfabrication Technology (Ministry of Education), Shanghai Jiao Tong University, Shanghai, China.
Bingbo BaoDepartment of Orthopedic Surgery, Shanghai Sixth People's Hospital Affiliated to Shanghai JiaoTong University School of Medicine, Shanghai, China.
Tianhui RenSchool of Chemistry and Chemical Engineering, Key Laboratory of Thin Film and Microfabrication Technology (Ministry of Education), Shanghai Jiao Tong University, Shanghai, China.
Yanan LiSchool of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai, China.
Yuan WangSchool of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai, China.
Sheng HanSchool of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai, China.
Yuan WangSchool of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai, China.
Xuan HuangDepartment of Orthopedics, The First Affiliated Hospital of Naval Military Medical University, Shanghai, China.
Hongxing HuDepartment of Orthopedic Surgery, Shanghai Sixth People's Hospital Affiliated to Shanghai JiaoTong University School of Medicine, Shanghai, China.ORCID https://orcid.org/0009-0001-0901-8720
Xianyou ZhengDepartment of Orthopedic Surgery, Shanghai Sixth People's Hospital Affiliated to Shanghai JiaoTong University School of Medicine, Shanghai, China.

Funding

Basic Medical Research Foundation of Naval Medical University 2023MS022Basic Medical Research Foundation of Naval Medical University 2023PY20Basic Medical Research Foundation of Naval Medical University 20XLS07：21QNPY039ChenGuang projectNational Natural Science Foundation of China 82372388Shanghai "Science and Technology Innovation Action Plan" Morning Star Cultivation 22YF1447500
6 · The paper itself

Abstract

Despite remarkable progress in total joint arthroplasty, aseptic loosening of titanium (Ti) alloy persists as a critical clinical challenge due to the poor wear resistance and biological inertness of such implants. Targeting of inflammatory osteolysis and remodelling of the osseointegration environment represent promising therapeutic approaches to address this issue. In this study, we developed a novel engineered extracellular vesicles (EVs) with a tag of dextran sulfate (DS-EVs) via metabolic glycan labelling (MGL)-mediated click chemistry. This targeted delivery of EVs, derived from metabolically engineered stem cells, establishes a new cell-free therapeutic system for periprosthetic osteolysis. DS-EVs demonstrated specific macrophage tropism, effectively reprogramming macrophage polarisation from pro-inflammatory M1 to regenerative M2 phenotypes. This phenotypic shift attenuated osteoclastogenesis while enhancing osseointegration through GPC6/Wnt pathway activation in vitro. Furthermore, we designed a multifunctional 3D titanium alloy scaffold with MXene-PVA composite hydrogel coatings (Ti-PPM scaffold). The multifunctional Ti-PPM composite scaffold, incorporating DS-EVs, provides a robust delivery system for periprosthetic osteolysis. This integrated system exhibits dual advantages of enhanced wear resistance and optimised interfacial adhesion, while enabling controlled EV release to maximize DS-EVs' osseointegration potential in vivo. Collectively, our findings establish DS-EVs as a transformative therapeutic modality for periprosthetic osteolysis through dual modulation of the osseointegration microenvironment and macrophage phenotypic heterogeneity.

Indexed as

Extracellular VesiclesHydrogelsOsteolysisAnimalsHumansMacrophagesMiceOsseointegrationOsteogenesisTissue ScaffoldsTitaniumHydrogelsTitaniumextracellular vesicleshydrogelmacrophageperiprosthetic osteolysistitanium alloys

Identifiers

PMID40545972
PMCPMC12183387

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.