Evidence map›Paper›PMID 40545606›Full record

ArticleEnvironmental and molecular mutagenesis2025

Identification of Hot and Cold Regions for Mutagenesis in the Escherichia coli tdk Gene: A Catalog of Mutational Sites.

Katherine Douglas, Dana Sorensen, Arnav Saud, Ananya Sridharan, Mallika Mathew, Jyotsna Hiranandani, Ava Gonick, Courtney Young, Kelly Nguyen, Nhu Phi and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Environmental and molecular mutagenesis, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Detection of mutations: from Ames test to duplex sequencing.Frontiers in molecular biosciences · 2026
    Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Katherine DouglasDepartment of Microbiology, Immunology, and Molecular Genetics, and the Molecular Biology Institute, University of California, and the David Geffen School of Medicine, Los Angeles, California, USA.
Dana SorensenDepartment of Microbiology, Immunology, and Molecular Genetics, and the Molecular Biology Institute, University of California, and the David Geffen School of Medicine, Los Angeles, California, USA.
Arnav SaudDepartment of Microbiology, Immunology, and Molecular Genetics, and the Molecular Biology Institute, University of California, and the David Geffen School of Medicine, Los Angeles, California, USA.
Ananya SridharanDepartment of Microbiology, Immunology, and Molecular Genetics, and the Molecular Biology Institute, University of California, and the David Geffen School of Medicine, Los Angeles, California, USA.
Mallika MathewDepartment of Microbiology, Immunology, and Molecular Genetics, and the Molecular Biology Institute, University of California, and the David Geffen School of Medicine, Los Angeles, California, USA.
Jyotsna HiranandaniDepartment of Microbiology, Immunology, and Molecular Genetics, and the Molecular Biology Institute, University of California, and the David Geffen School of Medicine, Los Angeles, California, USA.
Ava GonickDepartment of Microbiology, Immunology, and Molecular Genetics, and the Molecular Biology Institute, University of California, and the David Geffen School of Medicine, Los Angeles, California, USA.
Courtney YoungDepartment of Microbiology, Immunology, and Molecular Genetics, and the Molecular Biology Institute, University of California, and the David Geffen School of Medicine, Los Angeles, California, USA.
Kelly NguyenDepartment of Microbiology, Immunology, and Molecular Genetics, and the Molecular Biology Institute, University of California, and the David Geffen School of Medicine, Los Angeles, California, USA.
Nhu PhiDepartment of Microbiology, Immunology, and Molecular Genetics, and the Molecular Biology Institute, University of California, and the David Geffen School of Medicine, Los Angeles, California, USA.
Jeffrey H MillerDepartment of Microbiology, Immunology, and Molecular Genetics, and the Molecular Biology Institute, University of California, and the David Geffen School of Medicine, Los Angeles, California, USA.

Funding

University of California
6 · The paper itself

Abstract

We constructed a catalog of mutational sites in the tdk gene of Escherichia coli that consists of 378 different base pair substitutions at 245 different sites (base pairs). This allows us to examine the tendency of different sub-regions of the gene to be more or less prone to mutations when compared with other sub-regions. We do this by recording the number of occurrences of close to 1100 mutations resulting from 9 different mutagens or mutators at each site within a rolling 20-site interval. We can define 5 mutationally prone regions (MPRs). Aligning the profile of MPRs in tdk next to those we previously described in the thyA gene results in a linear map with 8 MPR regions, reinforcing the concept that certain sub-regions of genes are more mutable than others, even when other variables are accounted for. The catalog should prove useful for future studies.

Indexed as

Escherichia coliEscherichia coli ProteinsMutagenesisMutationEscherichia coli Proteinsbacterial systemsmutational hotspotstdk

Identifiers

PMID40545606
PMCPMC12451230

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.