Evidence map›Paper›PMID 40545375›Full record

ArticleThe Journal of veterinary medical science2025

Characterization of α-hemolysin-specific monoclonal antibodies for detection in the culture supernatant of pathogenic Escherichia coli strains.

Haruki Fujimoto, Sayaka Kida, Tomoko Kohda, Masafumi Mukamoto, Masahiro Kusumoto, Hideyuki Arimitsu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of veterinary medical science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Haruki FujimotoLaboratory of Microbiology, School of Human Science and Environment, University of Hyogo, Hyogo, Japan.
Sayaka KidaLaboratory of Microbiology, School of Human Science and Environment, University of Hyogo, Hyogo, Japan.
Tomoko KohdaGraduate School of Veterinary Science, Osaka Metropolitan University, Osaka, Japan.
Masafumi MukamotoGraduate School of Veterinary Science, Osaka Metropolitan University, Osaka, Japan.
Masahiro KusumotoGraduate School of Veterinary Science, Osaka Metropolitan University, Osaka, Japan.
Hideyuki ArimitsuLaboratory of Microbiology, School of Human Science and Environment, University of Hyogo, Hyogo, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

α-Hemolysin (α-Hly) is produced by pathogenic Escherichia coli and is implicated in a variety of diseases. In the swine, enterotoxigenic E. coli (ETEC) and Shiga toxin-producing E. coli (STEC) strains cause post-weaning diarrhea and edema disease, respectively, and most of these strains are hemolytic. However, the role of α-Hly in ETEC and STEC infection is unclear. Here, we prepared α-Hly-specific monoclonal antibodies by immunizing animals with one-third of the carboxy end of recombinant α-Hly. The four α-Hly-specific antibodies obtained reacted with α-Hly in the culture supernatant of clinical isolates in enzyme-linked immunosorbent assay and western blot analysis. In addition, because these four monoclonal antibodies inhibited E. coli-induced hemolysis, these antibodies might be useful tools for investigating the significance of α-Hly in pathogenic strains.

Indexed as

Antibodies, MonoclonalEnterotoxigenic Escherichia coliEscherichia coliEscherichia coli InfectionsEscherichia coli ProteinsHemolysin ProteinsShiga-Toxigenic Escherichia coliAnimalsBlotting, WesternEnzyme-Linked Immunosorbent AssayHemolysisMiceMice, Inbred BALB CSwineSwine DiseasesAntibodies, MonoclonalEscherichia coli ProteinsHemolysin ProteinsHlya protein, E coliEscherichia colimonoclonal antibodyα-hemolysin

Identifiers

PMID40545375
PMCPMC12344312

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.