Evidence map›Paper›PMID 40543036›Full record

ArticleSTAR protocols2025

Protocol for cell transplantation in Nematostella vectensis, a Hexacorallian model.

Shani Talice, Shany K Barkan, Grace A Snyder, Itamar Kozlovski, Ton Sharoni, Tom Levy, Magda Lewandowska, David Ehrens, Eliya Sultan, Alona Azarov and 9 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Shani TaliceThe Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, Center for Regenerative Medicine and Stem Cells, Ben Gurion University of the Negev, Beer Sheva 8410501, Israel; The Goldman Sonnenfeldt School of Sustainability and Climate Change, Ben Gurion University of the Negev, Beer Sheva 8410501, Israel.
Shany K BarkanThe Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, Center for Regenerative Medicine and Stem Cells, Ben Gurion University of the Negev, Beer Sheva 8410501, Israel; The Goldman Sonnenfeldt School of Sustainability and Climate Change, Ben Gurion University of the Negev, Beer Sheva 8410501, Israel.
Grace A SnyderThe Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, Center for Regenerative Medicine and Stem Cells, Ben Gurion University of the Negev, Beer Sheva 8410501, Israel; Rosenstiel School of Marine, Atmospheric, and Earth Science, University of Miami, Miami, FL 33149, USA.
Itamar KozlovskiDepartment of Ecology, Evolution and Behavior, Alexander Silberman Institute of Life Sciences, Faculty of Science, The Hebrew University of Jerusalem, Jerusalem 9190401, Israel.
Ton SharoniDepartment of Ecology, Evolution and Behavior, Alexander Silberman Institute of Life Sciences, Faculty of Science, The Hebrew University of Jerusalem, Jerusalem 9190401, Israel.
Tom LevyInstitute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Hopkins Marine Station, Stanford University, Pacific Grove, CA 93950, USA.
Magda LewandowskaDepartment of Ecology, Evolution and Behavior, Alexander Silberman Institute of Life Sciences, Faculty of Science, The Hebrew University of Jerusalem, Jerusalem 9190401, Israel.
David EhrensRosenstiel School of Marine, Atmospheric, and Earth Science, University of Miami, Miami, FL 33149, USA.
Eliya SultanThe Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, Center for Regenerative Medicine and Stem Cells, Ben Gurion University of the Negev, Beer Sheva 8410501, Israel.
Alona AzarovThe Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, Center for Regenerative Medicine and Stem Cells, Ben Gurion University of the Negev, Beer Sheva 8410501, Israel; The Goldman Sonnenfeldt School of Sustainability and Climate Change, Ben Gurion University of the Negev, Beer Sheva 8410501, Israel.
Shelly OisherThe Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, Center for Regenerative Medicine and Stem Cells, Ben Gurion University of the Negev, Beer Sheva 8410501, Israel.
Ronnie YannaiThe Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, Center for Regenerative Medicine and Stem Cells, Ben Gurion University of the Negev, Beer Sheva 8410501, Israel.
Reuven AharoniDepartment of Ecology, Evolution and Behavior, Alexander Silberman Institute of Life Sciences, Faculty of Science, The Hebrew University of Jerusalem, Jerusalem 9190401, Israel.
Uzi HadadIlse Katz Institute for Nanoscale Science and Technology, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel.
Claytus DavisThe Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, Center for Regenerative Medicine and Stem Cells, Ben Gurion University of the Negev, Beer Sheva 8410501, Israel.
Yehu MoranDepartment of Ecology, Evolution and Behavior, Alexander Silberman Institute of Life Sciences, Faculty of Science, The Hebrew University of Jerusalem, Jerusalem 9190401, Israel.
Orly Gershoni-YahalomThe Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, Center for Regenerative Medicine and Stem Cells, Ben Gurion University of the Negev, Beer Sheva 8410501, Israel.
Nikki Traylor-KnowlesRosenstiel School of Marine, Atmospheric, and Earth Science, University of Miami, Miami, FL 33149, USA.
Benyamin RosentalThe Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, Center for Regenerative Medicine and Stem Cells, Ben Gurion University of the Negev, Beer Sheva 8410501, Israel. Electronic address: rosentab@post.bgu.ac.il.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Stem cell transplantation is a promising strategy for regenerating damaged tissues in Hexacorallia, a cnidarian subclass that includes stony corals threatened by anthropogenic stressors. Here, we present a protocol for cell transplantation in Nematostella vectensis, a Hexacorallian model. We describe steps for chemotherapy treatment, cell dissociation, and transplantation. We then detail procedures for assessment via fluorescence microscopy and flow cytometry. This technique lays the groundwork for potential coral restoration and conservation applications. For complete details on the use and execution of this protocol, please refer to Talice et al.

Indexed as

Sea AnemonesStem Cell TransplantationAnimalsAnthozoaFlow CytometryMicroscopy, FluorescenceCell-based AssaysCell isolationEvolutionary biologyFlow CytometryImmunologyModel Organisms

Identifiers

PMID40543036
PMCPMC12221732

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.