ArticleActa biochimica et biophysica Sinica2025
5'-tiRNA-Lys maintains intestinal epithelial homeostasis by EWSR1-dependent suppression of miR-125a and autophagy activation.
Article in Acta biochimica et biophysica Sinica, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Canonical pathways and selective mechanisms of autophagy in inflammatory bowel disease.Advanced biotechnology · 2026Review
- Anti-Inflammatory and Protective Role of tiRNA-Glu-TTC-003 in Pediatric Sepsis Via TREM2/TLR4 Signaling Modulation.Inflammation · 2026Article
- Serum small RNA profiling identifies prognostic biomarkers for sepsis mortality prediction.Frontiers in medicine · 2025Article
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Authors and funding
8 authors.
Funding
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Abstract
The intestinal epithelium relies on autophagy to maintain barrier integrity and homeostasis, and dysregulation of this process has been implicated in inflammatory bowel disease (IBD). While tRNA-derived small RNAs (tsRNAs) are emerging as regulators of cellular stress responses, their roles in intestinal autophagy remain poorly understood. Here, we identify that 5'-tiRNA-Lys, a tsRNA derived from mature tRNA-Lys, is significantly upregulated in the inflamed intestinal epithelium of IBD patients. Overexpression of 5'-tiRNA-Lys in mice ameliorates dextran sulfate sodium (DSS)-induced colitis. Mechanistically, 5'-tiRNA-Lys enhances autophagy in intestinal epithelial cells (IECs), as evidenced by elevated LC3-II level and autophagosome formation. RNA pull-down and immunoprecipitation assays reveal that 5'-tiRNA-Lys directly binds to the RNA-binding protein EWSR1 via its RNA recognition motif, disrupting EWSR1's interaction with the Drosha/DGCR8 microprocessor complex. This interference specifically suppresses the maturation of miR-125a, a microRNA that targets the autophagy-promoting gene
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