Production and biochemical characterization of Pleurotus ostreatus NRC 620 laccase and evaluation of its efficacy in apple juice clarification.

Ali M Elshafei, Maysa A Elsayed, Gamil E Ibrahim, Nayra S Mehanna, Mohamed M Hassan, Abdelmageed M Othman

Abstract read

In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Green and novel cleaning method by plasma-activated water on a microfiltration membrane after apple juice clarification.Food chemistry: X · 2026
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Ali M ElshafeiMicrobial Chemistry Department, Biotechnology Research Institute, National Research Centre, 33 El Bohouth St., Dokki, Giza, 12622, Egypt.

Maysa A ElsayedMicrobial Chemistry Department, Biotechnology Research Institute, National Research Centre, 33 El Bohouth St., Dokki, Giza, 12622, Egypt.

Gamil E IbrahimChemistry of Flavor and Aroma Department, Food Industries and Nutrition Research Institute, National Research Centre, 33 El Bohouth St., Dokki, Giza, 12622, Egypt.

Nayra S MehannaDairy Sciences Department, Food Industries and Nutrition Research Institute, National Research Centre, 33 El Bohouth St., Dokki, Giza, 12622, Egypt.

Mohamed M HassanMicrobial Chemistry Department, Biotechnology Research Institute, National Research Centre, 33 El Bohouth St., Dokki, Giza, 12622, Egypt.

Abdelmageed M OthmanMicrobial Chemistry Department, Biotechnology Research Institute, National Research Centre, 33 El Bohouth St., Dokki, Giza, 12622, Egypt. othmanam_nrc@yahoo.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The highest activity of the Pleurotus ostreatus NRC620 laccase enzyme occurred in the broth of mushroom growth after 25 days of incubation at 28 °C and static conditions. The optimum pH and temperature of the enzyme activity were revealed at pH 3.0, and 70 °C, respectively, and retained 68.33 and 59.61% of its activity after incubation at 40 and 50 °C for 2 h, respectively. The enzyme retained 100% activity after 2 h of incubation in citrate-phosphate buffer (pH 7.0). The addition of MgSO

Indexed as

Fruit and Vegetable JuicesLaccaseMalusPleurotusEnzyme StabilityHydrogen-Ion ConcentrationKineticsPhenolsTemperatureLaccasePhenolsBiochemical characterizationJuice clarificationLaccasePleurotus ostreatus

Identifiers

PMID40542046

DOI10.1016/j.biortech.2008.01.084

PMCPMC12181291

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.