Evidence map›Paper›PMID 40542045›Full record

ArticleScientific reports2025

Attention-driven UNet enhancement for accurate segmentation of bacterial spore outgrowth in microscopy images.

Saqib Qamar, Dmitry Malyshev, Rasmus Öberg, Daniel P G Nilsson, Magnus Andersson

Abstract read
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Saqib QamarDepartment of Physics, Umeå University, 90187, Umeå, Sweden.
Dmitry MalyshevDepartment of Physics, Umeå University, 90187, Umeå, Sweden.
Rasmus ÖbergDepartment of Physics, Umeå University, 90187, Umeå, Sweden.
Daniel P G NilssonDepartment of Physics, Umeå University, 90187, Umeå, Sweden.
Magnus AnderssonDepartment of Physics, Umeå University, 90187, Umeå, Sweden. magnus.andersson@umu.se.

Funding

Kempestiftelserna JCK-2129.3NAISS 2022-06725
6 · The paper itself

Abstract

Analyzing microscopy images of large growing cell samples using traditional methods is a complex and time-consuming process. In this work, we have developed an attention-driven UNet-enhanced model using deep learning techniques to efficiently quantify the position, area, and circularity of bacterial spores and vegetative cells from images containing more than 10,000 bacterial cells. Our attention-driven UNet algorithm has an accuracy of 96%, precision of 82%, sensitivity of 81%, and specificity of 98%. Therefore, it can segment cells at a level comparable to manual annotation. We demonstrate the efficacy of this model by applying it to a live-dead decontamination assay. The model is provided in three formats: Python code, a Binder that operates within a web browser without needing installation, and a Flask Web application for local use.

Indexed as

Image Processing, Computer-AssistedMicroscopySpores, BacterialAlgorithmsDeep LearningContaminationDeep learningSpores

Identifiers

PMID40542045
PMCPMC12181384

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.