ArticleBiosensors & bioelectronics2025
Multiplexed profiling of single-cell secretion on a hierarchical loading microwell chip.
Article in Biosensors & bioelectronics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
2 citing papers in PubMed.
- Review
- A high-throughput modular microfluidic platform for versatile functional assays at single-cell level.Microsystems & nanoengineering · 2026Article
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Authors and funding
3 authors.
Funding
Abstract
Protein secretion is involved in many biological processes, such as cellular communication, embryonic development, tissue homeostasis, disease pathogenesis, and execution of immune functions. Highly multiplexed detection of single-cell secretion is greatly needed but has always been challenging. Herein, we present a hierarchical loading microwell chip (HL-Chip) -based method that efficiently aligns thousands of single cells with multiple antibody-coated microbeads for highly multiplexed detection of secreted proteins from single cells. We demonstrate the applications of this platform in profiling secretion of six cytokines from single T cells and macrophages after pan- and antigen-specific stimulation. The 6-plex cytokine profiling reveals an early but transient cytokine burst and polyfunctional heterogeneity in antigen peptide-stimulated T cell receptor-engineered T (TCR-T) cells. This simple and versatile technology could find its application in single-cell measurements in both biological discoveries and clinical diagnosis.
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Registered trials
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