Evidence map›Paper›PMID 40540047›Full record

ArticleBioprocess and biosystems engineering2025

Production of copper nanoparticles using genetically engineered Komagataella phaffii.

Cancan Dong, Fan Wu, Shufan Liu, Youyan Rong, Kai Hong, Yumeng Li, Jiao Meng, Xin Wu

Abstract read
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In one paragraph

Article in Bioprocess and biosystems engineering, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Cancan Dong *State Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Science, Tianjin, 300308, China.
Fan Wu *State Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Science, Tianjin, 300308, China.
Shufan LiuState Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Science, Tianjin, 300308, China.
Youyan RongState Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Science, Tianjin, 300308, China.
Kai HongState Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Science, Tianjin, 300308, China.
Yumeng LiState Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Science, Tianjin, 300308, China.
Jiao MengState Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Science, Tianjin, 300308, China. jiaomeng123@yeah.net.
Xin WuState Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Science, Tianjin, 300308, China. wuxin@tib.cas.cn.

Funding

the National key R&D program of China 2021YFD1301002Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project TSBICIP-CXRC-031
6 · The paper itself

Abstract

With the increasing application of copper nanoparticles (CuNPs) as antibacterial agents, numerous studies have emerged in recent years focusing on their preparation and utilization. However, the existing physical and chemical processes for CuNPs synthesis are complex and environmentally hazardous, creating a demand for greener alternatives. Komagataella phaffii has been recognized as a cost-effective system for metal biosorption. Nevertheless, high concentrations of heavy metal particles inhibit cell growth and result in low biosorption efficiency of metal-based nanoparticles (NPs). To address this issue, we engineered the K. phaffii strain X-33-Cyb5R by expressing the cytochrome b-5 reductase (Cyb5R) enzyme, enhancing its tolerance to elevated heavy metal concentrations and promoting CuNPs biosorption. Through further optimization of biosorption conditions, CuNPs production reached 14.27 mg/g dry cell weight (DCW) after 36 h, utilizing 12 mmol/L CuSO

Indexed as

Anti-Bacterial AgentsCopperGenetic EngineeringMetal NanoparticlesSaccharomycetalesAnti-Bacterial AgentsCopperAntibacterialCu biosorptionCytochrome b-5 reductaseKomagataella phaffiiNanoparticlesPichia pastoris

Identifiers

PMID40540047

What OpenQuestion holds

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