Evidence map›Paper›PMID 40539096›Full record

ArticleFrontiers in bioengineering and biotechnology2025

Generation of decellularized human brain tissue for investigating cell-matrix interactions: a proof-of-concept study.

Roemel Jeusep Bueno, Camila Fernández-Zapata, Maren Salla, Juliana Campo Garcia, Aylin Alacam, Oliver Klein, Chotima Böttcher, Helena Radbruch, Friedemann Paul, Sarah C Starossom and 2 more

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in bioengineering and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors.

Roemel Jeusep BuenoExperimental and Clinical Research Center, A Cooperation Between Max Delbrück Center and Charité Universitätsmedizin Berlin, Berlin, Germany.
Camila Fernández-ZapataExperimental and Clinical Research Center, A Cooperation Between Max Delbrück Center and Charité Universitätsmedizin Berlin, Berlin, Germany.
Maren SallaMax-Delbrück-Center for Molecular Medicine (MDC), Berlin Institute for Medical Systems Biology (BIMSB), Berlin, Germany.
Juliana Campo GarciaExperimental and Clinical Research Center, A Cooperation Between Max Delbrück Center and Charité Universitätsmedizin Berlin, Berlin, Germany.
Aylin AlacamExperimental and Clinical Research Center, A Cooperation Between Max Delbrück Center and Charité Universitätsmedizin Berlin, Berlin, Germany.
Oliver KleinBerlin Institute of Health (BIH), Center for Regenerative Therapies, Berlin, Germany.
Chotima BöttcherExperimental and Clinical Research Center, A Cooperation Between Max Delbrück Center and Charité Universitätsmedizin Berlin, Berlin, Germany.
Helena RadbruchDepartment of Neuropathology, Charité-Universitätsmedizin, Berlin, Germany.
Friedemann PaulExperimental and Clinical Research Center, A Cooperation Between Max Delbrück Center and Charité Universitätsmedizin Berlin, Berlin, Germany.
Sarah C StarossomExperimental and Clinical Research Center, A Cooperation Between Max Delbrück Center and Charité Universitätsmedizin Berlin, Berlin, Germany.
Rafaela V Silva *Experimental and Clinical Research Center, A Cooperation Between Max Delbrück Center and Charité Universitätsmedizin Berlin, Berlin, Germany.
Carmen Infante-Duarte *Experimental and Clinical Research Center, A Cooperation Between Max Delbrück Center and Charité Universitätsmedizin Berlin, Berlin, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The brain extracellular matrix (ECM) regulates myelin repair and regeneration following a demyelinating event by interacting with neuronal progenitors and immune cells. Therefore, generation and characterization of decellularized human brain tissue (DHBT) in regions with distinct neuroregenerative capacities are essential to determine factors modulating the cellular regenerative behavior. We have established an effective decellularization protocol for the human neural stem cell (NSC)-rich subventricular zone (SVZ) as well as, frontal cortex (FC) and white matter (WM), and defined region-specific matrisomes with comparative proteomics. Subsequently, as proof-of-concept, survival and differentiation of NSCs and monocytes within the DHBT were investigated. The proteomic analysis of the DHBT confirmed the retention of matrisome proteins such as COL4A1, FBB, NCAN, ANXA2. Unique to the SVZ were LGI3 and C1QB, while annexins, S100A and TGM2 were found in FC; S100B was exclusive to the WM. NSCs cultured within WM and FC acquired an astrocytic phenotype, but both astrocytic and oligodendrocytic phenotypes were promoted by the SVZ DHBT. Moreover, imaging mass cytometry analysis indicated an anti-inflammatory phenotype differentiation of monocytes seeded on SVZ and WM. Thus, the established model is suitable for investigation of ECM properties and assessment of cell-matrix interactions.

Indexed as

brain extracellular matrixbrain proteomicsimaging mass cytometrymatrisomemonocytesneuronal stem cells

Identifiers

PMID40539096
PMCPMC12177465

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.