Evidence map›Paper›PMID 40538929›Full record

ArticleFrontiers in pediatrics2025

The value of DHR-enzyme-linked immunosorbent assay in the diagnosis of chronic granulomatous disease by detecting NADPH oxidase complex activity.

Wen Xiaohui, Zhao Shunying, Zhang Xiaoyan, Tang Xiaolei, Shen Yuelin, Liu Jinrong, Li Huimin, Liu Hui, Yang Haiming

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in pediatrics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Wen XiaohuiDepartment No. 2 of Respiratory Medicine, National Children's Medical Center, Beijing Children's Hospital, Capital Medical University, Beijing, China.
Zhao ShunyingDepartment No. 2 of Respiratory Medicine, National Children's Medical Center, Beijing Children's Hospital, Capital Medical University, Beijing, China.
Zhang XiaoyanDepartment No. 2 of Respiratory Medicine, National Children's Medical Center, Beijing Children's Hospital, Capital Medical University, Beijing, China.
Tang XiaoleiDepartment No. 2 of Respiratory Medicine, National Children's Medical Center, Beijing Children's Hospital, Capital Medical University, Beijing, China.
Shen YuelinDepartment No. 2 of Respiratory Medicine, National Children's Medical Center, Beijing Children's Hospital, Capital Medical University, Beijing, China.
Liu JinrongDepartment No. 2 of Respiratory Medicine, National Children's Medical Center, Beijing Children's Hospital, Capital Medical University, Beijing, China.
Li HuiminDepartment No. 2 of Respiratory Medicine, National Children's Medical Center, Beijing Children's Hospital, Capital Medical University, Beijing, China.
Liu HuiDepartment No. 2 of Respiratory Medicine, National Children's Medical Center, Beijing Children's Hospital, Capital Medical University, Beijing, China.
Yang HaimingDepartment No. 2 of Respiratory Medicine, National Children's Medical Center, Beijing Children's Hospital, Capital Medical University, Beijing, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: A rapid, easy, and accurate method for screening Chronic Granulomatous Disease (CGD) is crucial. This study aimed to propose and evaluate the effectiveness of the DHR-Enzyme-linked immunosorbent assay (DHR-ELISA) for assessing NADPH oxidase complex activity in the early screening of CGD. Methods: We conducted a retrospective analysis of 72 children suspected of having CGD who underwent NADPH oxidase activity assessment and genetic testing at Beijing Children's Hospital between July 2015 and January 2022. Results: Of the subjects, 57 were male and 15 were female, resulting in a male-to-female ratio of 3.8:1. The median age at onset was 6 months, and the median age at diagnosis was 15 months. Thirty-eight patients were diagnosed with CGD based on typical clinical manifestations and genetic testing, exhibiting symptoms such as left lymphadenopathy or calcification (65.8%), a large Calmette-Guérin scar (60.5%), a history of skin or other lymph node infections (52.6%), and specific pulmonary infections (23.7%). Thirty-one patients exhibited normal enzyme activity, whereas 41 showed reduced activity. The DHR-ELISA method demonstrated a specificity of 90% and a sensitivity ranging from 90.5% to 100% in detecting CGD. Conclusion: The DHR-ELISA is a rapid, easy, cost-effective, and efficient method for screening CGD, making it suitable for early diagnosis and potentially improving prognosis.

Indexed as

childrenchronic granulomatous diseaseDHR-enzyme-linked immunosorbent assayNADPH oxidase complex activity detectionrespiratory burst

Identifiers

PMID40538929
PMCPMC12176808

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