Evidence map›Paper›PMID 40537478›Full record

ArticleNPJ biofilms and microbiomes2025

Genomic evidence for flies as carriers of zoonotic pathogens on dairy farms.

Andrew J Sommer, Joseph H Skarlupka, Serafino Teseo, Saria Otani, Garret Suen, Kerri L Coon, Panagiotis Sapountzis

Abstract read
In one paragraph

Article in NPJ biofilms and microbiomes, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Flies from meat processing facilities are carriers of multidrug-resistant Escherichia coli and diverse Staphylococcaceae species.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Andrew J SommerDepartment of Bacteriology, University of Wisconsin-Madison, Madison, WI, USA.ORCID http://orcid.org/0000-0001-7798-6435
Joseph H SkarlupkaDepartment of Bacteriology, University of Wisconsin-Madison, Madison, WI, USA.
Serafino TeseoINRAE-UCA, UMR0454 MEDIS, Clermont-Ferrand, France.
Saria OtaniNational Food Institute, Technical University of Denmark, Kongens Lyngby, Denmark.
Garret SuenDepartment of Bacteriology, University of Wisconsin-Madison, Madison, WI, USA.ORCID http://orcid.org/0000-0002-6170-711X
Kerri L CoonDepartment of Bacteriology, University of Wisconsin-Madison, Madison, WI, USA. kerri.coon@wisc.edu.ORCID http://orcid.org/0000-0002-2701-5195
Panagiotis SapountzisINRAE-UCA, UMR0454 MEDIS, Clermont-Ferrand, France. panagiotis.sapountzis@inrae.fr.ORCID http://orcid.org/0000-0001-6286-3918

Funding

United States Department of Agriculture | National Institute of Food and Agriculture (NIFA) WIS04039
6 · The paper itself

Abstract

Dairy farms are major reservoirs of zoonotic bacterial pathogens, which harbor antimicrobial resistance genes (ARGs), and raise critical questions about their dissemination on and off the farm environment. Here, we investigated the role of coprophagous muscid flies (Diptera: Muscidae) as carriers of zoonotic pathogens and antimicrobial resistance. We collected cow manure and flies on a dairy farm and used shotgun metagenomics to identify the presence of clinically relevant bacteria, virulence factors, and ARGs in both environments. Our results reveal that, although the fly microbiome is largely composed of manure-associated taxa, they also harbor specific insect-associated bacteria, which may be involved in nutrient provisioning to the host. Furthermore, we identifed shared ARGs, virulence factors, and zoonotic pathogens enriched within the fly gastrointestinal tract (GIT). Our study illustrates the potential flow of pathogenic microorganisms from manure to coprophagous flies, suggesting that flies may pose an important zoonotic threat on dairy farms.

Indexed as

BacteriaMuscidaeZoonosesAnimalsCattleDairyingDrug Resistance, BacterialFarmsGenomicsHumansManureMetagenomicsVirulence FactorsManureVirulence Factors

Identifiers

PMID40537478
PMCPMC12179284

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.