Evidence map›Paper›PMID 40536976›Full record

ArticleScience (New York, N.Y.)2025

Lineage-resolved analysis of embryonic gene expression evolution in

Christopher R L Large, Rupa Khanal, LaDeana Hillier, Chau Huynh, Connor Kubo, Junhyong Kim, Robert H Waterston, John I Murray

Abstract read
In one paragraph

Article in Science (New York, N.Y.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Transcriptomic profiling of the embryonicbioRxiv : the preprint server for biology · 2026
    Article
  7. A gap-free, telomere-to-telomere genome assembly for thebioRxiv : the preprint server for biology · 2026
    Article
  8. Article
  9. Article
  10. Article
  11. Review
  12. Review
  13. Article
  14. Article
  15. High-resolution single-cell analyses reveal evolutionary constraints and evolvability of sexual circuits inProceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  16. A gene expression atlas of a juvenile nervous system.bioRxiv : the preprint server for biology · 2025
    Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Christopher R L LargeDepartment of Genetics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.ORCID 0000-0002-5732-970X
Rupa KhanalDepartment of Genetics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
LaDeana HillierDepartment of Genome Sciences, University of Washington, Seattle, WA, USA.
Chau HuynhDepartment of Genome Sciences, University of Washington, Seattle, WA, USA.ORCID 0009-0006-4211-2811
Connor KuboDepartment of Genome Sciences, University of Washington, Seattle, WA, USA.ORCID 0000-0001-9579-2917
Junhyong KimDepartment of Biology, University of Pennsylvania, Philadelphia, PA, USA.ORCID 0000-0002-7726-8246
Robert H WaterstonDepartment of Genome Sciences, University of Washington, Seattle, WA, USA.ORCID 0000-0001-5956-5215
John I MurrayDepartment of Genetics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.ORCID 0000-0003-4026-584X

Funding

Resource ProjectU41HG007355 · NHGRI · UNIVERSITY OF WASHINGTON · PI WATERSTON, ROBERT H · 2013 to 2021
$20.8M
Comparing cell types across mutants and species at single cell resolutionR01HD105819 · NICHD · UNIVERSITY OF PENNSYLVANIA · PI MURRAY, JOHN ISAAC · 2021 to 2025
$2.2M
Gene expression and regulation in C. elegans with single cell resolutionR01HG010478 · NHGRI · UNIVERSITY OF WASHINGTON · PI WATERSTON, ROBERT H · 2019 to 2021
$1.5M
NHGRI NIH HHS R01 HG010478NHGRI NIH HHS U41 HG007355NICHD NIH HHS R01 HD105819
6 · The paper itself

Abstract

The constraints that govern the evolution of gene expression patterns across development remain unclear. Single-cell RNA sequencing can detail these constraints by systematically profiling homologous cells. The conserved invariant embryonic lineage of

Indexed as

CaenorhabditisEvolution, MolecularGene Expression Regulation, DevelopmentalAnimalsCaenorhabditis elegansCell LineageEmbryonic DevelopmentEmbryo, NonmammalianGene Expression ProfilingNeuronsSingle-Cell AnalysisTranscriptome

Identifiers

PMID40536976
PMCPMC12327058

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.