Evidence map›Paper›PMID 40536564›Full record

ArticleApplied microbiology and biotechnology2025

Establishing a co-culture aggregate of N-cycle bacteria to elucidate flocculation in biological wastewater treatment.

Laurens Parret, Kenneth Simoens, Benjamin Horemans, Jo De Vrieze, Ilse Smets

Abstract read
In one paragraph

Article in Applied microbiology and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Laurens ParretDepartment of Chemical Engineering, Bio- & Chemical Reactor Engineering and Safety (CREaS), KU Leuven, Celestijnenlaan 200F box 2424, B-3001, Leuven, Belgium.ORCID http://orcid.org/0000-0002-4157-7709
Kenneth SimoensDepartment of Chemical Engineering, Bio- & Chemical Reactor Engineering and Safety (CREaS), KU Leuven, Celestijnenlaan 200F box 2424, B-3001, Leuven, Belgium.ORCID http://orcid.org/0000-0002-7967-4213
Benjamin HoremansDepartment of Chemical Engineering, Bio- & Chemical Reactor Engineering and Safety (CREaS), KU Leuven, Celestijnenlaan 200F box 2424, B-3001, Leuven, Belgium.ORCID http://orcid.org/0000-0001-6980-3715
Jo De VriezeCentre for Microbial Ecology and Technology (CMET), Ghent University, Frieda Saeysstraat 1, B-9052, Gent, Belgium.ORCID http://orcid.org/0000-0001-9365-8896
Ilse SmetsDepartment of Chemical Engineering, Bio- & Chemical Reactor Engineering and Safety (CREaS), KU Leuven, Celestijnenlaan 200F box 2424, B-3001, Leuven, Belgium. ilse.smets@kuleuven.be.ORCID http://orcid.org/0000-0001-8570-5568

Funding

Fonds Wetenschappelijk Onderzoek FWO- 1191022NFonds Wetenschappelijk Onderzoek FWO-G032321NKU Leuven C24/18/043
6 · The paper itself

Abstract

Biological flocculation is a complex phenomenon that is often treated as a black box. As a result, flocculation problems are usually remediated without knowledge of the exact causes. We show that it is feasible to exploit a model (N-cycle) consortium with reduced complexity to fundamentally study bioflocculation. Strong nitrifier microcolonies were formed during oxic/anoxic cycles in sequencing batch reactors, using alginate entrapment as a cell retention system. After the release of these aggregates into suspension, macroclusters with flocs of the denitrifier were observed. These results suggest that a living model of a full-scale activated sludge floc can be built through the use of this bottom-up approach. By eliminating shifts in the microbial community, the applied experimental conditions have a more direct effect on the observations. Key Points

Indexed as

BacteriaNitrogenWastewaterWater PurificationAlginatesBioreactorsCoculture TechniquesFlocculationMicrobial ConsortiaNitrificationSewageAlginatesNitrogenSewageWastewaterAlginateBioflocculationFISHNitrifiersPure culturesqPCR

Identifiers

PMID40536564
PMCPMC12178987

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.