Evidence map›Paper›PMID 40536399›Full record

ArticleJournal of cellular physiology2025

In Old Mice, Exercise Induces Inflammation and Fibrosis Unless Alk5-Inhibitor and Oxytocin Are Used.

Joana Marie C Cruz, Hayden Yeung, Rana Alzalzalee, Qile Yang, Hannaneh Kabir, Samantha Annaliese McDonough, Xiaoyue Mei, Michael J Conboy, Irina M Conboy

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cellular physiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Joana Marie C CruzDepartment of Bioengineering and QB3 Institute, University of California Berkeley, Berkeley, California, USA.ORCID 0000-0002-6896-4119
Hayden YeungDepartment of Bioengineering and QB3 Institute, University of California Berkeley, Berkeley, California, USA.ORCID 0009-0007-2372-7842
Rana AlzalzaleeDepartment of Bioengineering and QB3 Institute, University of California Berkeley, Berkeley, California, USA.
Qile YangDepartment of Bioengineering and QB3 Institute, University of California Berkeley, Berkeley, California, USA.ORCID 0009-0005-0148-2499
Hannaneh KabirDepartment of Bioengineering and QB3 Institute, University of California Berkeley, Berkeley, California, USA.
Samantha Annaliese McDonoughDepartment of Bioengineering and QB3 Institute, University of California Berkeley, Berkeley, California, USA.
Xiaoyue MeiDepartment of Bioengineering and QB3 Institute, University of California Berkeley, Berkeley, California, USA.
Michael J ConboyDepartment of Bioengineering and QB3 Institute, University of California Berkeley, Berkeley, California, USA.ORCID 0000-0002-6434-4772
Irina M ConboyDepartment of Bioengineering and QB3 Institute, University of California Berkeley, Berkeley, California, USA.

Funding

Identifying signatures of brain aging through heterochronic blood exchangeR01AG071787 · NIA · UNIVERSITY OF CALIFORNIA SANTA CRUZ · PI MESSERSMITH, PHILLIP B, MOURRAIN, PHILIPPE · 2021 to 2025
$2.3M
GABAergic Cells in the Periaqueductal Gray Region Control Food-SeekingR01DK139605 · NIDDK · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Avishek Adhikari · 2024 to 2026
$1.6M
NIA NIH HHS R01 AG071787NIDDK NIH HHS R01 DK139605
6 · The paper itself

Abstract

Exercise and diet are the best-known methods for attenuating aging-related health decline. However, exercise in older age has diminished gains of strength and agility, and a danger of unrepaired muscle damage. Improving the understanding of age-related differences in response to exercise, our results demonstrate that in old mice, downhill treadmill (eccentric) exercise causes increased influx of CD45+ cells (inflammation) and fibrotic index (fibrosis) in the heart and skeletal muscles. To explain these changes, we identified newly synthesized proteins through bio-orthogonal noncanonical amino acid tagging (BONCAT) and established that exercise exacerbated age-associated protein patterns through a dysregulated transforming growth factor (TGF)-β, Ras/MAPK/PI3Akt, and JAK/STAT pathways. Testing causality, we found that an inhibitor of TGF-β (Alk5 inhibitor, A5i) in combination with the age-diminished peptide oxytocin, previously shown to rejuvenate muscle and brain in sedentary animals, allowed aged mice to exercise without pathologies of skeletal and heart muscles and youthfully restored their de novo proteomes.

Indexed as

AgingInflammationOxytocinPhysical Conditioning, AnimalReceptor, Transforming Growth Factor-beta Type IAnimalsFibrosisMaleMiceMice, Inbred C57BLMuscle, SkeletalMyocardiumSignal TransductionTransforming Growth Factor betaOxytocinReceptor, Transforming Growth Factor-beta Type ITgfbr1 protein, mouseTransforming Growth Factor betaagingexercisefibrosisinflammationoxytocinproteomeTGF‐beta

Identifiers

PMID40536399
PMCPMC12178232

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.