Zebrafish caudal fin model to investigate the role of Cissus quadrangularis, bioceramics, and tendon extracellular matrix scaffolds in bone regeneration.

Introduction: Periodontal bone regeneration faces several challenges such as incomplete bone-ligament-cementum restoration, unpredictable clinical outcomes and membrane exposure risks. The zebra fish caudal fin model of regeneration could be an excellent model system for mimicking the periodontal regenerative criteria and the present study aimed to explore the potential of innovative composite scaffolds characterized by Materials and methods: Zebrafish were subjected to caudal fin amputation, followed by the application of different scaffold groups. The fabricated scaffolds were then crosslinked using glutaraldehyde vapours and stored in a desiccator for future use. The scaffolds were classified into five groups: negative control, PERIO COL- GTR (G1), Results: The physical characterization showed the scaffold interaction by spectral differentiation between the groups. Among the groups, Test group 4 demonstrated superior regeneration with enhanced collagen deposition, reduced inflammatory response, and significantly higher growth compared to control. Conclusion: These findings suggested that group 4 scaffold significantly accelerates zebrafish fin regeneration, making these scaffolds promising candidates for periodontal tissue engineering and regenerative medicine applications.