Evidence map›Paper›PMID 40535542›Full record

ArticleFrontiers in cellular and infection microbiology2025

Establishment and epidemiological investigation of a dual fluorescent qPCR assay for

Yao Pan, Qingting Yu, Qi Wang, Qiang Li, Wei Tian, Lingxiang Xin, Xing Hu, Haiyue Xiao, Yuanjie Liu, Luo Rong Deng Zhu and 3 more

Abstract read
In one paragraph

Article in Frontiers in cellular and infection microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Yao PanAnimal Husbandry Science Institute of Ganzi Tibetan Autonomous Prefecture, Kangding, China.
Qingting YuAnimal Husbandry Science Institute of Ganzi Tibetan Autonomous Prefecture, Kangding, China.
Qi WangChina Institute of Veterinary Drug Control, Beijing, China.
Qiang LiAnimal Husbandry Science Institute of Ganzi Tibetan Autonomous Prefecture, Kangding, China.
Wei TianAnimal Husbandry Science Institute of Ganzi Tibetan Autonomous Prefecture, Kangding, China.
Lingxiang XinChina Institute of Veterinary Drug Control, Beijing, China.
Xing HuAnimal Husbandry Science Institute of Ganzi Tibetan Autonomous Prefecture, Kangding, China.
Haiyue XiaoAnimal Husbandry Science Institute of Ganzi Tibetan Autonomous Prefecture, Kangding, China.
Yuanjie LiuChina Institute of Veterinary Drug Control, Beijing, China.
Luo Rong Deng ZhuAnimal Husbandry Science Institute of Ganzi Tibetan Autonomous Prefecture, Kangding, China.
Lan LanAnimal Husbandry Science Institute of Ganzi Tibetan Autonomous Prefecture, Kangding, China.
Liangquan ZhuChina Institute of Veterinary Drug Control, Beijing, China.
Jianping WuAnimal Husbandry Science Institute of Ganzi Tibetan Autonomous Prefecture, Kangding, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Yaks serve as a vital economic and ecological resource in high-altitude regions, but it faces significant health challenges from various pathogens. Among these, Methods: A duplex real-time fluorescence quantitative PCR assay was developed to simultaneously detect Results: The established assay demonstrated high sensitivity, with detection limits of 100 and 10 copies for pMD-kmt1 and pMD-invA, respectively. No cross-reactivity was observed with six pathogens, including Discussion: This study successfully established a duplex real-time fluorescence PCR assay that enables the simultaneous detection of

Indexed as

Cattle DiseasesPasteurella InfectionsPasteurella multocidaReal-Time Polymerase Chain ReactionSalmonellaSalmonella Infections, AnimalAnimalsBacterial ProteinsCattleChinaDNA PrimersSensitivity and SpecificityTibetBacterial ProteinsDNA PrimersinvA protein, Bacteriadetectionduplex real-time fluorescence PCRPasteurella multocidaSalmonellayak

Identifiers

PMID40535542
PMCPMC12174084

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.