ArticleACS ES&T water2025
Isolation and Molecular Identification of Pathogenic Free-Living Amoebae from Environmental Samples in Tenerife, Canary Islands, Spain.
Article in ACS ES&T water, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- High Occurrence of Pathogenic Free-Living Amoebae in Arid Environments.Pathogens (Basel, Switzerland) · 2025Article
- Molecular Species Identification and Genotyping of Free-Living Amoebae in Soil of Recreational Mountain Areas in the Babiogórski National Park and Surroundings, Southern Poland.International journal of molecular sciences · 2025Article
- High Diversity and Prevalence of Potentially Pathogenic Free-Living Amoebae in Water Sources from Castilla y León, Spain.Pathogens (Basel, Switzerland) · 2025Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Free-living amoebae (FLA) are protozoa that have been reported worldwide from various environmental sources. However, only some groups (Acanthamoeba spp., Naegleria fowleri, Balamuthia mandrillaris, Sappinia pedata, Vahlkampfia spp., and Vermamoeba vermiformis) are considered to be pathogenic for humans and other animals. FLA has been previously reported in the Canary Islands archipelago since 2005. However, climate change and other environmental factors that could affect the communities of FLA in the islands lead to the development of this extra study. This study was carried out in Tenerife, the largest and most populated island, between June 2021 and July 2022. A total of 28 soil samples and 23 water samples were seeded in 2% non-nutrient agar (NNA) plates, incubated at 26 °C, and monitored daily to evaluate the presence of FLA. DNA was extracted from those plates on which there was suspected FLA growth, and polymerase chain reaction amplification of the 18S rRNA gene (DF3• region on the case of Acanthamoeba) was carried out. The obtained results showed that the genus Acanthamoeba (25/51:48.07%), with the genotype T4 being the most common (21/25; 84%), and the species V. vermiformis (7/51; 13.73%) were the most abundant FLA in the surveyed samples collected from Tenerife.
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