ArticlePractical laboratory medicine2025
Direct identification of Mycobacterium species from liquid medium (MGIT) using FastPrep-2 bead beating system and MALDI-TOF mass spectrometry technology: a comparison with solid media and PCR-based method.
Article in Practical laboratory medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed.
- Clinical peptidomics for respiratory diseases: matrices, workflows, and translation towards treatable traits, with a focus on COPD.Clinical proteomics · 2026Review
- Advanced diagnostic methods for nontuberculous mycobacterial infections.Frontiers in tuberculosis · 2026Review
- Direct identification of Mycobacterium species from liquid medium (MGIT) using FastPrep-2 bead beating system and MALDI-TOF mass spectrometry technology: a comparison with solid media and PCR-based method.Practical laboratory medicine · 2025Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: Mycobacterial infections present significant global health challenges. Traditional diagnostic methods are inadequate for the identification of Mycobacterium species, highlighting the need for more efficient techniques. Matrix-assisted laser desorption-ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) technology offers potential advantages in rapid and accurate pathogen identification. Objective: This study evaluates the accuracy and precision of the MALDI-TOF using the FastPrep-2 bead beating system and VITEK MS version 3.2 for identifying Mycobacterium species directly from Mycobacteria Growth Indicator Tube (MGIT), liquid medium, compared to MALDI-TOF (VITEK MS) based on the traditional solid medium (Lowenstein-Jensen). We also compared the result of the MALDI-TOF from MGIT to Methods: The study included 16 Results: NTM comprised 70 % (37/53) of the total isolates evaluated. Conclusion: Using VITEK MS version 3.2 to directly identify MTB and NTM from MGIT liquid medium provides a rapid, cost-effective, and reliable method for identifying Mycobacterium species. Further optimization and database expansion are recommended to enhance accuracy for rare and less common mycobacterial species.
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Registered trials
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