Evidence map›Paper›PMID 40533478›Full record

ArticleNature communications2025

Reversible proliferative arrest induced by rapid depletion of RNase MRP.

Yuan Liu, Shiyang He, Kawon Pyo, Reuben Franklin, Ibrahim B Maaz, Chen Cai, Kriti Shah, Sihem Cheloufi, William F Marzluff, Jernej Murn

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Yuan Liu *Department of Biochemistry, University of California, Riverside, 3401 Watkins Drive, Boyce Hall, Riverside, CA, USA.
Shiyang He *Department of Biochemistry, University of California, Riverside, 3401 Watkins Drive, Boyce Hall, Riverside, CA, USA.ORCID http://orcid.org/0000-0002-1849-2635
Kawon PyoDepartment of Biochemistry, University of California, Riverside, 3401 Watkins Drive, Boyce Hall, Riverside, CA, USA.ORCID http://orcid.org/0009-0004-4001-384X
Reuben FranklinDepartment of Biochemistry, University of California, Riverside, 3401 Watkins Drive, Boyce Hall, Riverside, CA, USA.ORCID http://orcid.org/0000-0002-6513-3205
Ibrahim B MaazDepartment of Biochemistry, University of California, Riverside, 3401 Watkins Drive, Boyce Hall, Riverside, CA, USA.
Chen CaiDepartment of Biochemistry, University of California, Riverside, 3401 Watkins Drive, Boyce Hall, Riverside, CA, USA.ORCID http://orcid.org/0009-0005-6964-9208
Kriti ShahDepartment of Biochemistry, University of California, Riverside, 3401 Watkins Drive, Boyce Hall, Riverside, CA, USA.
Sihem CheloufiDepartment of Biochemistry, University of California, Riverside, 3401 Watkins Drive, Boyce Hall, Riverside, CA, USA. sihem.cheloufi@ucr.edu.ORCID http://orcid.org/0000-0002-1726-4796
William F MarzluffIntegrated Program for Biological and Genome Sciences, University of North Carolina, Chapel Hill, North Carolina, USA. william_marzluff@med.unc.edu.ORCID http://orcid.org/0000-0003-3635-4073
Jernej MurnDepartment of Biochemistry, University of California, Riverside, 3401 Watkins Drive, Boyce Hall, Riverside, CA, USA. jernej.murn@ucr.edu.ORCID http://orcid.org/0000-0002-1729-5859

Funding

CONTROL OF HISTONE MRNA SYNTHESISR01GM029832 · NIGMS · UNIV OF NORTH CAROLINA CHAPEL HILL · PI DOMINSKI, ZBIGNIEW, MARZLUFF, WILLIAM F. · 1985 to 2022
$9.0M
Investigating the molecular mechanisms of early neuronal morphogenesisR01GM144693 · NIGMS · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI Jernej Murn · 2022 to 2026
$1.7M
Investigating the Regulatory Roles of Histone Chaperones in Cellular PlasticityR35GM151004 · NIGMS · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI Sihem Cheloufi · 2023 to 2026
$1.6M
National Science Foundation (NSF) 2054195NIGMS NIH HHS R01 GM029832NIGMS NIH HHS R01 GM144693NIGMS NIH HHS R35 GM151004U.S. Department of Health & Human Services | National Institutes of Health (NIH) GM29832
6 · The paper itself

Abstract

Cellular quiescence is a state of reversible proliferative arrest that plays essential roles in development, resistance to stress, aging, and longevity of organisms. Here we report that rapid depletion of RNase MRP, a deeply conserved RNA-based enzyme required for rRNA biosynthesis, induces a long-term yet reversible proliferative arrest in human cells. Severely compromised biogenesis of rRNAs along with acute transcriptional reprogramming precede a gradual decline of the critical cellular functions. Unexpectedly, many arresting cells show increased levels of histone mRNAs, which accumulate locally in the cytoplasm, and S-phase DNA amount. The ensuing proliferative arrest is entered from multiple stages of the cell cycle and can last for several weeks with uncompromised cell viability. Strikingly, restoring expression of RNase MRP leads to a complete reversal of the arrested state with resumed cell proliferation at the speed of control cells. We suggest that targeting rRNA biogenesis may provide a general strategy for rapid induction of a reversible proliferative arrest, with implications for understanding and manipulating cellular quiescence.

Indexed as

Cell Cycle CheckpointsCell ProliferationEndoribonucleasesHistonesHumansRNA, MessengerRNA, RibosomalEndoribonucleasesHistonesmitochondrial RNA-processing endoribonucleaseRNA, MessengerRNA, Ribosomal

Identifiers

PMID40533478
PMCPMC12177063

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.