ArticleNature communications2025
Enzymatic twists evolved stereo-divergent alkaloids in the Solanaceae family.
Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
6 citing papers in PubMed.
- Loss, persistence and reversal of phenotypic traits.Biological reviews of the Cambridge Philosophical Society · 2026Review
- Biosynthetic mechanism and ecological function of chirality in Solanum steroidal alkaloids.Nature communications · 2026Article
- Rewiring Steroidal Metabolic Pathways for Diosgenin Production in Solanum nigrum.Plant biotechnology journal · 2026Article
- Complex engineering of Solanum alkaloids structural diversity in Nicotiana benthamiana.Plant biotechnology journal · 2026Article
- Convergence and constraint in glucosinolate evolution across the Brassicaceae.The Plant cell · 2025Article
- Tomato secondary metabolites as natural regulators ofFrontiers in plant science · 2025Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
Abstract
Steroidal alkaloids play a crucial role in plant defense and exhibit distinct stereochemistry at C25, forming either the tomato-type (25S) or eggplant-type (25R) isomers. Here, we uncover the molecular mechanisms shaping this stereochemical diversity. Phylogenetic analysis of GLYCOALKALOID METABOLISM 8 (GAME8) cytochrome P450 hydroxylases across the Solanaceae family revealed two distinct clades producing either 25S or 25R isomers. Ancestral GAME8 likely favored 25R, with gene duplications giving rise to 25S-producing enzymes in more recent Solanum species. In S. nigrum and S. dulcamara, multiple GAME8 copies generate mixed isomeric profiles. Notably, in wild S. cheesmaniae from the Galápagos, mutations in GAME8 have driven a shift from 25S back to the ancestral 25R, suggesting reverse evolution. Our findings highlight how GAME8 evolution has shaped alkaloid diversity in the genus Solanum, demonstrating a complex interplay between enzyme function, genetic variation, and evolutionary adaptation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.