ArticleScience advances2025
Tuning hydrogen bonds and electrostatics with convection for purifying mRNA: A paradigm shift.
Article in Science advances, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Integrated Purification and Desalting of Plasmid DNA Using Monolith Columns.Electrophoresis · 2026Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The increasing clinical trials of single-stranded mRNA (ss-mRNA) therapeutics highlight the urgent need to develop efficient, scalable, and economic purification methods. Current diffusion-driven, resin-based purification techniques constrain productivity and rely on expensive oligo(dT) ligands for target ss-mRNA poly(A) tail hybridization. To overcome these challenges, we use interfacial molecular forces, such as charge and hydrogen bonds, between nucleic acid variants and a positively charged synthetic microporous membrane to purify ss-mRNA, a desirable therapeutic, from an undesirable impurity, immunogenic double-stranded RNA (dsRNA). Membranes achieved high binding capacities (1.28 mg/m
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