ArticleMemorias do Instituto Oswaldo Cruz2025
Prevalence of pfhrp2/pfhrp3 gene deletions among patients with Plasmodium falciparum malaria with false-negative in the HRP2-based rapid diagnostic test in Colombia.
Article in Memorias do Instituto Oswaldo Cruz, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Are pfhrp2 and pfhrp3 Deletions a Concern in Latin America? A Critical Review.Pathogens (Basel, Switzerland) · 2026Review
- Clinical and epidemiological profiles of confirmed malaria cases in the state of Pernambuco, Brazil, from 2001 to 2022.Revista da Sociedade Brasileira de Medicina Tropical · 2025Observational
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Authors and funding
6 authors.
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Abstract
backgroundIn malaria-endemic regions, rapid diagnostic tests (RDTs) play a crucial role in promptly identifying infections, especially in remote areas with limited microscopy services.
objectivesConduct a cross-sectional, multi-site study to determine whether the local prevalence of mutations in the Plasmodium falciparum hrp2/3 genes in false-negative RDTs has reached a threshold that might require a local or national change in diagnostic strategy in accordance with the WHO guidelines (2018).
methodsIndividuals were screened for P. falciparum with microscopy and HRP2-based RDT at health facilities. Discordant results between these two tests triggered diagnostic confirmation by polymerase chain reaction (PCR) and detection of the pfhrp2/pfhrp3 genes.
findingsAmong the 347 patients included, false negatives constituted 4.61% (16/347). Molecular analysis revealed all 16 false negatives were P. falciparum positive with hrp2 gene present, displaying high polymorphism. However, hrp3 gene deletion was observed in 93.8% (15/16) of these cases. MAIN
conclusionsThe prevalence of false-negative RDTs is low, and these results were not linked to deletions in the hrp2 gene. This suggests that there is no immediate need to modify the RDTs used along the Colombian Pacific Coast. However, molecular surveillance for hrp2 deletions remains crucial to detect any potential increase in prevalence.
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