Evidence map›Paper›PMID 40531469›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Automated Synthesis and Purification of Hydrophobic Peptides.

Wenbin Huang, Istvan Toth, Mariusz Skwarczynski

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Wenbin HuangSchool of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD, Australia.
Istvan TothSchool of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD, Australia.
Mariusz SkwarczynskiSchool of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD, Australia. m.skwarczynski@uq.edu.au.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Poly(hydrophobic amino acids) have been utilized as a component of peptide-based vaccines due to their ability to act as an immunostimulating moiety. They are biodegradable, possess defined stereochemistry and number of units, and upon conjugation to peptide antigens can self-assemble into nanoparticles. However, the process of synthesis and purification of the conjugates presents notable difficulties due to the highly hydrophobic nature of poly(hydrophobic amino acids), resulting in low production yield. Herein we present a protocol for the optimized automated synthesis and purification of these conjugates.

Indexed as

PeptidesAutomationHydrophobic and Hydrophilic InteractionsPeptidesFmoc-solid phase peptide synthesisHigh-yieldHydrophobic peptide synthesisPeptide-based vaccinePoly-leucineSelf-assembly

Identifiers

PMID40531469

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.