Optimizing the Synthesis of Desotamides and Related Cyclic Antimicrobial Peptides.

Archi Bansal, Yuzhang Li, Zyta M Ziora, Prashant Mishra, Peter M Moyle

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Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

5 authors.

Archi BansalThe University of Queensland and Indian Institute of Technology Delhi (UQ-IITD) Research Academy, St Lucia, Queensland, Australia.

Yuzhang LiSchool of Pharmacy, The University of Queensland, Woolloongabba, QLD, Australia.

Zyta M ZioraThe University of Queensland and Indian Institute of Technology Delhi (UQ-IITD) Research Academy, St Lucia, Queensland, Australia.

Prashant MishraThe University of Queensland and Indian Institute of Technology Delhi (UQ-IITD) Research Academy, St Lucia, Queensland, Australia.

Peter M MoyleThe University of Queensland and Indian Institute of Technology Delhi (UQ-IITD) Research Academy, St Lucia, Queensland, Australia. p.moyle@uq.edu.au.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The protocol described in this chapter details the synthesis of Desotamides, a class of cyclic hexapeptide antibiotics from Streptomyces species. These peptides, which often include D-amino acids and non-proteogenic amino acids, are challenging to produce using molecular biology techniques. Their synthesis involves loading the first amino acid onto 2-chlorotrityl chloride resin, followed by fluorenylmethyloxycarbonyl (Fmoc) solid phase synthesis of the linear peptide, cleavage of a protected peptide fragment from the resin using mild acidic conditions, and head-to-tail cyclization in solution. These synthetic processes permit the scalable production and engineering of Desotamides and novel-related antimicrobial species, without the limitations of biological synthesis methods.

Indexed as

Antimicrobial PeptidesPeptides, CyclicSolid-Phase Synthesis TechniquesCyclizationFluorenesStreptomycesAntimicrobial PeptidesFluorenesPeptides, CyclicAntimicrobialsPeptide cyclizationSolution phase cyclization

Identifiers

PMID40531466

DOI10.1016/j.tet.2004.01.020

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.