Evidence map›Paper›PMID 40531459›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Synthesis of Caged Aminoluciferin Peptides.

Daan Sondag, Thomas J Boltje, Floris P J T Rutjes

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Daan SondagInstitute for Molecules and Materials, Radboud University, Nijmegen, The Netherlands.
Thomas J BoltjeInstitute for Molecules and Materials, Radboud University, Nijmegen, The Netherlands.
Floris P J T RutjesInstitute for Molecules and Materials, Radboud University, Nijmegen, The Netherlands. floris.rutjes@ru.nl.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Luminescent imaging has become a pivotal technique for monitoring enzymatic activity, offering a high signal-to-noise ratio by avoiding external light sources. Traditional methods of coupling aminoluciferin to peptides have faced challenges due to the instability of the aminoluciferin. We have developed a solid-phase peptide synthesis (SPPS) protocol involving anchoring of P

Indexed as

Firefly LuciferinPeptidesSolid-Phase Synthesis TechniquesFirefly LuciferinPeptidesActivity-based probesEnzymatic activityGuanidinylationLuminescent imagingSerine proteasesSolid phase peptide synthesis (SPPS)

Identifiers

PMID40531459

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.